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Literature summary for 3.5.4.2 extracted from
- Purine salvage in Methanocaldococcus jannaschii Elucidating the role of a conserved cysteine in adenine deaminase (2016), Proteins, 84, 828-840 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanocaldococcus jannaschii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular docking studies. Substrate adenine is unable to properly orient in the active site in the C127A and C127S mutant structures due to distinct differences in active site conformation and rotation of residue D261 | Methanocaldococcus jannaschii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C127A | 10fold decrease in kcat over the wild-type | Methanocaldococcus jannaschii |
| C127S | complete loss of activity | Methanocaldococcus jannaschii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.92 | - |
adenine | wild-type, pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii |  |
| 2.4 | - |
adenine | mutant C127A, pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | purified enzyme contains 1.5 mol of Fe, 1.1 mol of Zn and 14 mol of Mn per mol. Enzyme uses either Fe(II) and/or Mn(II) as the catalytic metal | Methanocaldococcus jannaschii | |
| Mn2+ | purified enzyme contains 1.5 mol of Fe, 1.1 mol of Zn and 14 mol of Mn per mol. Enzyme uses either Fe(II) and/or Mn(II) as the catalytic metal | Methanocaldococcus jannaschii | |
| Zn2+ | purified enzyme contains 1.5 mol of Fe, 1.1 mol of Zn and 14 mol of Mn per mol | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
| Methanocaldococcus jannaschii DSM 2661 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.4 | - |
no addition of divalent cation, pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii |
| 5.3 | - |
presence of Fe2+, pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii |
| 5.8 | - |
presence of Mn2+, pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + H2O | - |
Methanocaldococcus jannaschii | hypoxanthine + NH3 | - |
? | |
| adenine + H2O | - |
Methanocaldococcus jannaschii DSM 2661 | hypoxanthine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MJ1459 | - |
Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
mutant C127S, stable up to | Methanocaldococcus jannaschii |
| 90 | - |
wild-type, stable up to | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22 | - |
adenine | mutant C127A, pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
| 221 | - |
adenine | wild-type, pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
adenine | mutant C127A, pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
| 240 | - |
adenine | wild-type, pH 7.0, temperature not specified in the publication | Methanocaldococcus jannaschii | |
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