Any feedback?
Literature summary for 3.5.4.2 extracted from
- Biochemical characterization of Kluyveromyces lactis adenine deaminase and guanine deaminase and their potential application in lowering purine content in beer (2018), Front. Bioeng. Biotechnol., 6, 180 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | in beer samples treated with adenine deaminase and guanine deaminase, the adenine concentration in beer drops 66-67% and guanine concentration in beer drops from 68.8 microM to a minimal amount | Kluyveromyces lactis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Kluyveromyces lactis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.68 | - |
adenine | pH 6.0, 30°C | Kluyveromyces lactis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
gel filtration | Kluyveromyces lactis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces lactis | - |
- |
- |
| Kluyveromyces lactis MTCC 458 | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 25 mM Tris, 100 mM NaCl, 15% glycerol and pH 6.0, stable for a month | Kluyveromyces lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-diaminopurine + H2O | - |
Kluyveromyces lactis | guanine + NH3 | - |
? | |
| 2,6-diaminopurine + H2O | - |
Kluyveromyces lactis MTCC 458 | guanine + NH3 | - |
? | |
| 6-chloropurine + H2O | - |
Kluyveromyces lactis | hypoxanthine + HCl | - |
? | |
| 6-chloropurine + H2O | - |
Kluyveromyces lactis MTCC 458 | hypoxanthine + HCl | - |
? | |
| adenine + H2O | preferred substrate | Kluyveromyces lactis | hypoxanthine + NH3 | - |
? | |
| adenine + H2O | preferred substrate | Kluyveromyces lactis MTCC 458 | hypoxanthine + NH3 | - |
? | |
| FAD + H2O | - |
Kluyveromyces lactis | ? | - |
? | |
| FAD + H2O | - |
Kluyveromyces lactis MTCC 458 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 41000, SDS-PAGE | Kluyveromyces lactis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADA | - |
Kluyveromyces lactis |
| KLLAOE2317 | - |
Kluyveromyces lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Kluyveromyces lactis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.1 | - |
adenine | pH 6.0, 30°C | Kluyveromyces lactis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Kluyveromyces lactis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | - |
Kluyveromyces lactis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
adenine | pH 6.0, 30°C | Kluyveromyces lactis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
