Literature summary for 3.5.4.17 extracted from

  • Yates, M.G.
    A non-specific adenine nucleotide deaminase from Desulfovibrio desulfuricans (1969), Biochim. Biophys. Acta, 171, 299-310.
    View publication on PubMed

General Stability

General Stability Organism
stable to several cycles of freezing and thawing Desulfovibrio desulfuricans

Inhibitors

Inhibitors Comment Organism Structure
2'-AMP inhibits deamination of ATP Desulfovibrio desulfuricans
3'-AMP inhibits deamination of ATP Desulfovibrio desulfuricans
adenosine inhibits deamination of ATP Desulfovibrio desulfuricans
Ag+ 0.5 mM, complete inhibition of deamination of ATP Desulfovibrio desulfuricans
beta,beta-dimethyl glutarate
-
Desulfovibrio desulfuricans
cacodylate
-
Desulfovibrio desulfuricans
citrate
-
Desulfovibrio desulfuricans
Cl-
-
Desulfovibrio desulfuricans
CN- non-competitive inhibition at low concentrations Desulfovibrio desulfuricans
Co2+
-
Desulfovibrio desulfuricans
CuSO4
-
Desulfovibrio desulfuricans
F- non-competitive inhibition at low concentrations Desulfovibrio desulfuricans
Fe3+
-
Desulfovibrio desulfuricans
Hg2+ 0.5 mM, complete inhibition of deamination of ATP Desulfovibrio desulfuricans
Maleate
-
Desulfovibrio desulfuricans
Mn2+
-
Desulfovibrio desulfuricans
phosphate
-
Desulfovibrio desulfuricans
p-mercuribenzoate
-
Desulfovibrio desulfuricans
succinate
-
Desulfovibrio desulfuricans
Urea 0.2-1 M Desulfovibrio desulfuricans
Zn2+
-
Desulfovibrio desulfuricans

Organism

Organism UniProt Comment Textmining
Desulfovibrio desulfuricans
-
var. azotovorans strain Berre S
-

Purification (Commentary)

Purification (Comment) Organism
-
Desulfovibrio desulfuricans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.4
-
-
Desulfovibrio desulfuricans

Storage Stability

Storage Stability Organism
-20°C, indefinitely stable Desulfovibrio desulfuricans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
Desulfovibrio desulfuricans inosine + NH3
-
ir
AMP + H2O
-
Desulfovibrio desulfuricans IMP + NH3
-
ir
ATP + H2O
-
Desulfovibrio desulfuricans ITP + NH3
-
ir
ADP + H2O
-
Desulfovibrio desulfuricans IDP + NH3
-
ir
NAD+ + H2O
-
Desulfovibrio desulfuricans nicotinamide-hypoxanthine dinucleotide + NH3
-
ir
deoxyadenosine + H2O
-
Desulfovibrio desulfuricans deoxyinosine + NH3
-
ir
dATP + H2O
-
Desulfovibrio desulfuricans dITP + NH3
-
ir
dADP + H2O
-
Desulfovibrio desulfuricans dIDP + NH3
-
ir
dAMP + H2O
-
Desulfovibrio desulfuricans dIMP + NH3
-
ir
ADP-ribose + H2O
-
Desulfovibrio desulfuricans IDP-ribose + NH3
-
ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
5 min, complete loss of activity Desulfovibrio desulfuricans
22
-
stable for 3 days at room temperature Desulfovibrio desulfuricans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5 deamination of ATP Desulfovibrio desulfuricans