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Literature summary for 3.5.4.16 extracted from

  • Schuessler, S.; Haase, I.; Perbandt, M.; Illarionov, B.; Siemens, A.; Richter, K.; Bacher, A.; Fischer, M.; Graewert, T.
    Structure of GTP cyclohydrolase I from Listeria monocytogenes, a potential anti-infective drug target (2019), Acta Crystallogr. Sect. F, 75, 586-592 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a recombinant Escherichia coli strain Listeria monocytogenes serotype 1/2a

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor-diffusion method at 20°C. Crystallized from 1.3 M sodium citrate pH 7.3. The crystal structure is solved at a resolution of 2.4 A Listeria monocytogenes serotype 1/2a

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
GTP pH and temperature not specified in the publication Listeria monocytogenes serotype 1/2a

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
SDS-PAGE Listeria monocytogenes serotype 1/2a

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Listeria monocytogenes serotype 1/2a
-
formate + 7,8-dihydroneopterin 3'-triphosphate
-
?
GTP + H2O Listeria monocytogenes serotype 1/2a ATCC BAA-679
-
formate + 7,8-dihydroneopterin 3'-triphosphate
-
?

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes serotype 1/2a Q8Y5X1
-
-
Listeria monocytogenes serotype 1/2a ATCC BAA-679 Q8Y5X1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Listeria monocytogenes serotype 1/2a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Listeria monocytogenes serotype 1/2a formate + 7,8-dihydroneopterin 3'-triphosphate
-
?
GTP + H2O
-
Listeria monocytogenes serotype 1/2a ATCC BAA-679 formate + 7,8-dihydroneopterin 3'-triphosphate
-
?

Subunits

Subunits Comment Organism
decamer 10 * 22000, SDS-PAGE Listeria monocytogenes serotype 1/2a

Synonyms

Synonyms Comment Organism
folE
-
Listeria monocytogenes serotype 1/2a