Cloned (Comment) | Organism |
---|---|
gene gch and adjacent regions, DNA and amino acid sequence determination and analysis, uncommon start codon TTG, ribosomal binding site, overexpression in Escherichia coli strains JM109 and BL21(DE3) as HAT-tagged enzyme, and expression in Mycobacterium smegmatis from plasmid | Nocardia iowensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
GTP | recombinant enzyme expressed in Escherichia coli | Nocardia iowensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24500 | - |
10 * 24563, sequence calculation, 10 * 24500, MALDI-TOF mass spectrometry, 10 * 35000, recombinant HAT-tagged enzyme | Nocardia iowensis |
24563 | - |
10 * 24563, sequence calculation, 10 * 24500, MALDI-TOF mass spectrometry, 10 * 35000, recombinant HAT-tagged enzyme | Nocardia iowensis |
35000 | - |
10 * 24563, sequence calculation, 10 * 24500, MALDI-TOF mass spectrometry, 10 * 35000, recombinant HAT-tagged enzyme | Nocardia iowensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Nocardia iowensis | first committed step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? | |
GTP + H2O | Nocardia iowensis NRRL 5646 | first committed step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia iowensis | Q8GLS8 | - |
- |
Nocardia iowensis NRRL 5646 | Q8GLS8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant HAT-tagged enzyme from Escherichia coli, 6.8fold | Nocardia iowensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of differently expressed recombinant enzymes | Nocardia iowensis |
0.000001 | - |
wild-type native enzyme and recombinant HAT10-tagged enzyme from expression in Escherichia coli BL21(DE3) | Nocardia iowensis |
0.000029 | - |
recombinant enzyme from expression in Mycobacterium smegmatis/pSMT-260 | Nocardia iowensis |
0.00027 | - |
recombinant HAT-tagged enzyme from expression in Escherichia coli JM109 | Nocardia iowensis |
0.00088 | - |
recombinant HAT-217-tagged enzyme from expression in Escherichia coli BL21(DE3) | Nocardia iowensis |
6 | - |
purified recombinant HAT-tagged enzyme expressed in Escherichia coli | Nocardia iowensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | first committed step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin | Nocardia iowensis | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? | |
GTP + H2O | complex series of reaction steps | Nocardia iowensis | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? | |
GTP + H2O | first committed step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin | Nocardia iowensis NRRL 5646 | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? | |
GTP + H2O | complex series of reaction steps | Nocardia iowensis NRRL 5646 | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 24563, sequence calculation, 10 * 24500, MALDI-TOF mass spectrometry, 10 * 35000, recombinant HAT-tagged enzyme | Nocardia iowensis |
More | digestion of recombinant HAT-tagged enzyme with enterokinase results in proteins of 29.5 and 32.5 kDa | Nocardia iowensis |
Synonyms | Comment | Organism |
---|---|---|
GCH | - |
Nocardia iowensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Nocardia iowensis |