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Literature summary for 3.5.4.16 extracted from

  • He, A.; Simpson, D.R.; Daniels, L.; Rosazza, J.P.
    Cloning, expression, purification, and characterization of Nocardia sp. GTP cyclohydrolase I (2004), Protein Expr. Purif., 35, 171-180.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gch and adjacent regions, DNA and amino acid sequence determination and analysis, uncommon start codon TTG, ribosomal binding site, overexpression in Escherichia coli strains JM109 and BL21(DE3) as HAT-tagged enzyme, and expression in Mycobacterium smegmatis from plasmid Nocardia iowensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
GTP recombinant enzyme expressed in Escherichia coli Nocardia iowensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
10 * 24563, sequence calculation, 10 * 24500, MALDI-TOF mass spectrometry, 10 * 35000, recombinant HAT-tagged enzyme Nocardia iowensis
24563
-
10 * 24563, sequence calculation, 10 * 24500, MALDI-TOF mass spectrometry, 10 * 35000, recombinant HAT-tagged enzyme Nocardia iowensis
35000
-
10 * 24563, sequence calculation, 10 * 24500, MALDI-TOF mass spectrometry, 10 * 35000, recombinant HAT-tagged enzyme Nocardia iowensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Nocardia iowensis first committed step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
?
GTP + H2O Nocardia iowensis NRRL 5646 first committed step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
?

Organism

Organism UniProt Comment Textmining
Nocardia iowensis Q8GLS8
-
-
Nocardia iowensis NRRL 5646 Q8GLS8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant HAT-tagged enzyme from Escherichia coli, 6.8fold Nocardia iowensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of differently expressed recombinant enzymes Nocardia iowensis
0.000001
-
wild-type native enzyme and recombinant HAT10-tagged enzyme from expression in Escherichia coli BL21(DE3) Nocardia iowensis
0.000029
-
recombinant enzyme from expression in Mycobacterium smegmatis/pSMT-260 Nocardia iowensis
0.00027
-
recombinant HAT-tagged enzyme from expression in Escherichia coli JM109 Nocardia iowensis
0.00088
-
recombinant HAT-217-tagged enzyme from expression in Escherichia coli BL21(DE3) Nocardia iowensis
6
-
purified recombinant HAT-tagged enzyme expressed in Escherichia coli Nocardia iowensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O first committed step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin Nocardia iowensis formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
?
GTP + H2O complex series of reaction steps Nocardia iowensis formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
?
GTP + H2O first committed step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin Nocardia iowensis NRRL 5646 formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
?
GTP + H2O complex series of reaction steps Nocardia iowensis NRRL 5646 formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
?

Subunits

Subunits Comment Organism
decamer 10 * 24563, sequence calculation, 10 * 24500, MALDI-TOF mass spectrometry, 10 * 35000, recombinant HAT-tagged enzyme Nocardia iowensis
More digestion of recombinant HAT-tagged enzyme with enterokinase results in proteins of 29.5 and 32.5 kDa Nocardia iowensis

Synonyms

Synonyms Comment Organism
GCH
-
Nocardia iowensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Nocardia iowensis