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Literature summary for 3.5.4.15 extracted from

  • Ishida, Y.; Shirafuji, H.; Kida, M.; Yoneda, M.
    Studies on the guanosine degrading system in bacterial cell. Part III. Purification and properties of guanosine deaminase (1969), Agric. Biol. Chem., 33, 384-390.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
4-Amino-5-imidazole carboxamide ribonucleoside
-
Pseudomonas putida
Al3+
-
Pseudomonas putida
Cd2+
-
Pseudomonas putida
Co2+
-
Pseudomonas putida
Cu2+
-
Pseudomonas putida
Hg2+
-
Pseudomonas putida
Mn2+
-
Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
guanosine
-
Pseudomonas putida
0.062
-
deoxyguanosine
-
Pseudomonas putida
0.122
-
8-azaguanosine
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000 20000 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanosine + H2O Pseudomonas putida
-
xanthosine + NH3
-
?
guanosine + H2O Pseudomonas putida No. 149
-
xanthosine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida No. 149
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyguanosine + H2O
-
Pseudomonas putida 2'-deoxyxanthosine + NH3
-
?
2'-deoxyguanosine + H2O
-
Pseudomonas putida No. 149 2'-deoxyxanthosine + NH3
-
?
8-azaguanosine + H2O
-
Pseudomonas putida 8-azaxanthosine + NH3
-
?
8-azaguanosine + H2O
-
Pseudomonas putida No. 149 8-azaxanthosine + NH3
-
?
guanosine + H2O
-
Pseudomonas putida xanthosine + NH3
-
?
guanosine + H2O
-
Pseudomonas putida No. 149 xanthosine + NH3
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9
-
Pseudomonas putida