Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | inhibition of deamination reaction | Methanocaldococcus jannaschii | |
dUTP | inhibition of deamination reaction | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | for dUTP, Km-value in millimolar range | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23300 | - |
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry | Methanocaldococcus jannaschii |
23400 | - |
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methanocaldococcus jannaschii | bifunctionality of enzyme avoids release of toxic UTP within cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
bifunctional enzyme dCTP deaminase-dUTP nucleotidohydrolase | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCTP + H2O | - |
Methanocaldococcus jannaschii | dUTP + NH3 | - |
? | |
ddCTP + H2O | deamination at the same rate as dCTP | Methanocaldococcus jannaschii | ddUTP + NH3 | - |
? | |
dUTP + 2 H2O | - |
Methanocaldococcus jannaschii | dUMP + 2 phosphate | - |
? | |
additional information | bifunctionality of enzyme avoids release of toxic UTP within cells | Methanocaldococcus jannaschii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23400, deduced from gene sequence, x * 23300, mass spectrometry | Methanocaldococcus jannaschii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
dTTP | pH 6.8, 25°C | Methanocaldococcus jannaschii | |
0.5 | - |
dUTP | - |
Methanocaldococcus jannaschii |