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Literature summary for 3.5.4.12 extracted from
- Chloroviruses encode a bifunctional dCMP-dCTP deaminase that produces two key intermediates in dTTP formation (2007), J. Virol., 81, 7662-7671.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dCTP | activates dCMP deamination about 7fold at 0.005-0.1 mM | Paramecium bursaria Chlorella virus 1 |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ORF A596R, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain DH5MCR, phylogenetic analysis | Paramecium bursaria Chlorella virus 1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dTTP | inhibits dCMP deamination, feedback inhibition | Paramecium bursaria Chlorella virus 1 | |
| H4dUMP | inhibits both dCTP and dCMP deaminase activities | Paramecium bursaria Chlorella virus 1 | |
| PDRP | inhibits both dCTP and dCMP deaminase activities | Paramecium bursaria Chlorella virus 1 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.64 | - |
dCTP | pH 9.5, 42°C | Paramecium bursaria Chlorella virus 1 | |
| 0.76 | - |
dCMP | pH 9.5, 42°C, in presence of 0.1 mM dCTP | Paramecium bursaria Chlorella virus 1 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially substitute for Mg2+ | Paramecium bursaria Chlorella virus 1 | |
| Mg2+ | the enzyme depends on divalent cations, Mg2+ is preferred, but can partially be substituted by Ca2+, Ni2+, or Mn2+ | Paramecium bursaria Chlorella virus 1 | |
| Mn2+ | can partially substitute for Mg2+ | Paramecium bursaria Chlorella virus 1 | |
| Ni2+ | can partially substitute for Mg2+ | Paramecium bursaria Chlorella virus 1 | |
| Zn2+ | the enzyme contains a zinc binding site, but shows less than 6% of maximal activity with Zn2+ as divalent cation | Paramecium bursaria Chlorella virus 1 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
6 * 17000, SDS-PAGE | Paramecium bursaria Chlorella virus 1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCMP + H2O | Paramecium bursaria Chlorella virus 1 | - |
dUMP + NH3 | dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis | ? | |
| dCTP + H2O | Paramecium bursaria Chlorella virus 1 | - |
dUTP + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paramecium bursaria Chlorella virus 1 | O41078 | i.e. PBCV-1 , a chlorovirus from host Chlorella sp. strain NC64A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, the protein quickly precipitates at concentration higher than 2 mg/ml | Paramecium bursaria Chlorella virus 1 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| the purified recombinant His-tagged enzyme in stable without precipitation for more than 1 year in 50% glycerol | Paramecium bursaria Chlorella virus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCMP + H2O | - |
Paramecium bursaria Chlorella virus 1 | dUMP + NH3 | - |
? | |
| dCMP + H2O | - |
Paramecium bursaria Chlorella virus 1 | dUMP + NH3 | dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis | ? | |
| dCTP + H2O | - |
Paramecium bursaria Chlorella virus 1 | dUTP + NH3 | - |
? | |
| additional information | the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 17000, SDS-PAGE | Paramecium bursaria Chlorella virus 1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dCMP-dCTP deaminase | - |
Paramecium bursaria Chlorella virus 1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Paramecium bursaria Chlorella virus 1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | - |
Paramecium bursaria Chlorella virus 1 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.7 | - |
dCTP | pH 9.5, 42°C | Paramecium bursaria Chlorella virus 1 | |
| 24.9 | - |
dCMP | pH 9.5, 42°C, in presence of 0.1 mM dCTP | Paramecium bursaria Chlorella virus 1 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
dCTP deaminase activity | Paramecium bursaria Chlorella virus 1 |
| 9.5 | - |
dCMP deaminase activity | Paramecium bursaria Chlorella virus 1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | activity range | Paramecium bursaria Chlorella virus 1 |
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