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Literature summary for 3.5.4.12 extracted from
- Modifications of deoxycytidine kinase and deaminase activities by docosahexaenoic acid in normal and transformed rat fibroblasts (2002), Biochem. Pharmacol., 63, 717-723.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| (4Z,7Z,10Z,13Z,16Z,19Z)-henicosa-4,7,10,13,16,19-hexaenoic acid | increases the enzyme activity in vivo in normal Rat-2 fibroblasts, but decreases it in transformed NM-16 fibroblasts | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (4Z,7Z,10Z,13Z,16Z,19Z)-henicosa-4,7,10,13,16,19-hexaenoic acid | decreases the enzyme activity in vivo in transformed NM-16 fibroblasts, but increases it in normal Rat-2 fibroblasts | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.347 | - |
deoxycytidine | pH 8.1, 37°C, Rat-2 fibroblasts in presence of 0.02 mM docosahexanoic acid | Rattus norvegicus | |
| 0.349 | - |
deoxycytidine | pH 8.1, 37°C, Rat-2 fibroblasts | Rattus norvegicus | |
| 0.462 | - |
deoxycytidine | pH 8.1, 37°C, NM-16 fibroblasts | Rattus norvegicus | |
| 0.467 | - |
deoxycytidine | pH 8.1, 37°C, NM-16 fibroblasts in presence of 0.02 mM docosahexanoic acid | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxycytidine + H2O | Rattus norvegicus | the enzyme is a key enzyme in the inactivation of deoxycytidine and several chemotherapeutically important nucleoside analogues | deoxyuridine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | the enzyme activity is higher in normal Rat-2 fibroblast compared to transformed NM-16 cells | Rattus norvegicus | - |
| NW-16 cell | transformed fibroblast cell line | Rattus norvegicus | - |
| Rat-2 cell | fibroblast cell line | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxycytidine + H2O | - |
Rattus norvegicus | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | the enzyme is a key enzyme in the inactivation of deoxycytidine and several chemotherapeutically important nucleoside analogues | Rattus norvegicus | deoxyuridine + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dCDA | - |
Rattus norvegicus |
| deoxycytidine deaminase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
assay at | Rattus norvegicus |
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