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Literature summary for 3.5.4.12 extracted from
- Properties of an affinity-column-purified human deoxycytidylate deaminase (1993), Biochim. Biophys. Acta, 1162, 161-170.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dCTP | allosteric activator | Homo sapiens |  |
| dCTP | positive allosteric effector | Homo sapiens | |
| dCTP | activation by dCTP is reversed by addition of dTTP | Homo sapiens | |
| dTTP | allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA from HeLa cells can be expressed in an active form | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Homo sapiens |
| dCTP and MgCl2 stabilize | Homo sapiens |
| enzyme extremely labile, stabilized by dCTP /Mg2+ | Homo sapiens |
| ethyleneglycol prevents protein denaturation during freezing in the presence of 2-mercaptoethanol | Homo sapiens |
| ethyleneglycol, 10-20% stabilizes | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-beta-D-deoxyribose-pyrimidin-2-one 5'-phosphate | competitive inhibition, Ki: 0.000012 mM | Homo sapiens | |
| 3,4,5,6-tetrahydro-2'-dUMP | potent inhibitor, Ki: 0.00001 mM | Homo sapiens | |
| 5-(acryloylamino-pentanol-1)2'-beta-D-dUMP | - |
Homo sapiens | |
| 5-Hg-dCMP | potent inhibitor, in the absence of 2-mercaptoethanol, 0.00024 mM: 37% inhibition, 0.0024 mM: 70% inhibition | Homo sapiens | |
| 5-Hg-dUMP | potent inhibitor, 0.0001 mM: 54% inhibition | Homo sapiens | |
| dAMP | - |
Homo sapiens | |
| dCTP | allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH | Homo sapiens | |
| dGMP | - |
Homo sapiens | |
| dTTP | 0.0015 mM: 50% inhibition. dTTP inhibition reversed by dCTP; allosteric inhibitor | Homo sapiens | |
| dUMP | - |
Homo sapiens | |
| Hg(CH3COO)2 | Hg(acetate)2; mercuroacetate, potent inhibitor, 0.0002 mM: 26% inhibition, 0.001 mM: 40% inhibition | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective | Homo sapiens | |
| Co2+ | divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective | Homo sapiens | |
| divalent cations | divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective | Homo sapiens | |
| Mg2+ | divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective | Homo sapiens | |
| Mg2+ | required for activation by dCTP and for inhibition by dTTP | Homo sapiens | |
| Mg2+ | required for activation by dCTP, Mg2+ in the absence of dCTP: no effect | Homo sapiens | |
| Mn2+ | divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective | Homo sapiens | |
| additional information | divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective | Homo sapiens | |
| Zn2+ | divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence | Homo sapiens |
| 20000 | - |
6 * 20000, SDS-PAGE | Homo sapiens |
| 117000 | - |
in the presence of dCTP and Mg2+, 4°C, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCMP + H2O | Homo sapiens | enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division | dUMP + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HeLa cells | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Homo sapiens | |
| dCMP + H2O = dUMP + NH3 | most dramatic allosteric response at substrate levels 0.1 mM dCMP or less | Homo sapiens | |
| dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 481 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10-20% ethyleneglycol, 0.01 mM dCTP, 1 mM MgCl2, 20 mM 2-mercaptoethanol and 0.05% Triton X-100, 1 year, no loss in activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-Hg-dCMP + H2O | very poor substrate in the absence of mercaptoethanol | Homo sapiens | 5-Hg-dUMP + NH3 | - |
? | |
| 5-Hg-dCMP + H2O | deamination activated by dTTP and inhibited by dCTP | Homo sapiens | 5-Hg-dUMP + NH3 | - |
? | |
| 5-Hg-dCMP + H2O | 5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol | Homo sapiens | 5-Hg-dUMP + NH3 | - |
? | |
| 5-hydroxymethyl-dCMP + H2O | HeLa cells, good substrate | Homo sapiens | 5-hydroxymethyl-dUMP + NH3 | - |
? | |
| 5-methyl-dCMP + H2O | HeLa cells, good substrate | Homo sapiens | 5-methyl-dUMP + NH3 | - |
? | |
| CMP + H2O | HeLa cells, at 1 mM, in presence and absence of dCTP and Mg2+: not a substrate | Homo sapiens | UMP + NH3 | - |
? | |
| dCMP + H2O | most dramatic allosteric response at substrate levels 0.1 mM dCMP or less | Homo sapiens | dUMP + NH3 | - |
? | |
| dCMP + H2O | enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division | Homo sapiens | dUMP + NH3 | - |
? | |
| additional information | substrate specificity | Homo sapiens | ? | - |
? | |
| additional information | HeLa cells, no activity with CDP at 1 mM, in presence and absence of dCTP and Mg2+ | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 identical subunits | Homo sapiens |
| hexamer | 6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence | Homo sapiens |
| hexamer | 6 * 20000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
optimum condition | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 960 | - |
dCMP | - |
Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | HeLa cells, in presence or absence of dCTP and Mg2+ | Homo sapiens |
| 8 | - |
assay at | Homo sapiens |
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