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Literature summary for 3.5.4.12 extracted from

  • Xu, Y.Z.; Plunkett, W.
    Modulation of deoxycytidylate deaminase in intact human leukemia cells. Action of 2',2'-difluorodeoxycytidine (1992), Biochem. Pharmacol., 44, 1819-1827.
    View publication on PubMed
Search for more literature for 3.5.4.12

Activating Compound

Activating Compound Comment Organism Structure
dCTP positive allosteric effector Homo sapiens

Application

Application Comment Organism
pharmacology elevated level of dCMPase in transformed cells and tumors: enzyme may represent another important target for cancer chemotherapy Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2',2'-difluoro-dCTP dual mechanism of inhibition; partial purified enzyme Homo sapiens
2',2'-difluorodeoxycytidine high cellular nucleotide levels: inhibition: Concentration-dependent inhibition; inhibition by nucleotides of 2',2'-difluorodeoxycytidine, dual mechanism of inhibition Homo sapiens
2'-deoxythymidine 0.05 mM: 60% inhibition, 0.01 mM: 5% inhibition; dThd Homo sapiens
3,4,5,6-tetrahydro-2'-deoxyuridine specifc inhibition Homo sapiens
3,4,5,6-tetrahydro-2'-dUMP specific potent inhibitor Homo sapiens
dTTP allosteric inhibitor Homo sapiens
additional information little or no inhibition by 3,4,5,6-tetrahydrouridine; mechanism of inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCMP + H2O Homo sapiens key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis dUMP + NH3
-
 ?
additional information Homo sapiens enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
partial, leukemia cells Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
dCMP + H2O = dUMP + NH3 mechanism Homo sapiens
a
dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Homo sapiens
a
dCMP + H2O = dUMP + NH3 feedback regulation Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
CCRF-CEM cell
-
 Homo sapiens
-
KG-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',2'-difluoro-dCMP + H2O
-
 Homo sapiens 2',2'-difluoro-dUMP + NH3
-
 ?
dCMP + H2O
-
 Homo sapiens dUMP + NH3
-
 ?
dCMP + H2O key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis Homo sapiens dUMP + NH3
-
 ?
additional information enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells Homo sapiens ?
-
 ?