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Literature summary for 3.5.4.12 extracted from
- T2r+ bacteriophage-induced enzymes. I. The purification and properties of deoxycytidylate deaminase (1972), J. Biol. Chem., 247, 931-939.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5-hydroxymethyl-dCTP | natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP | Tequatrovirus T4 |  |
| 5-hydroxymethyl-dCTP | natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP | Escherichia phage T2 | |
| 5-hydroxymethyl-dCTP | pH-dependent activation | Tequatrovirus T4 | |
| 5-hydroxymethyl-dCTP | pH-dependent activation | Escherichia phage T2 | |
| dCTP | positive allosteric effector | Gallus gallus | |
| dCTP | positive allosteric effector | Tequatrovirus T4 | |
| dCTP | positive allosteric effector | Escherichia phage T2 | |
| dCTP | activation by dCTP decreases markedly as the pH is increased, pH-dependent activation | Escherichia phage T2 | |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Tequatrovirus T4 |
| 2-mercaptoethanol stabilizes | Escherichia phage T2 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dTTP | allosteric inhibitor; inhibition by dTTP increases markedly as the pH is raised, pH-dependent inhibition | Escherichia phage T2 | |
| dTTP | allosteric inhibitor | Gallus gallus | |
| dTTP | allosteric inhibitor | Tequatrovirus T4 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
dCMP | pH 6.0 | Escherichia phage T2 | |
| 0.1 | - |
dCMP | pH 8.0 | Escherichia phage T2 | |
| 0.5 | - |
CMP | pH 6.0 | Escherichia phage T2 | |
| 12 | - |
CMP | pH 8.0, absence of effector | Escherichia phage T2 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 124000 | - |
PAGE and sedimentation equilibrium | Escherichia phage T2 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCMP + H2O | Gallus gallus | major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway | dUMP + NH3 | - |
? | |
| dCMP + H2O | Tequatrovirus T4 | major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway | dUMP + NH3 | - |
? | |
| dCMP + H2O | Escherichia phage T2 | major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway | dUMP + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia phage T2 | - |
T2r+ or T4r+ bacteriophage-induced enzyme, host Escherichia coli B | - |
| Gallus gallus | - |
- |
- |
| Tequatrovirus T4 | - |
T2r+ or T4r+ bacteriophage-induced enzyme, host Escherichia coli B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| T2-phage infected Escherichia coli | Escherichia phage T2 |
| T4-phage infected Escherichia coli | Tequatrovirus T4 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Gallus gallus | |
| dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Tequatrovirus T4 | |
| dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Escherichia phage T2 | |
| dCMP + H2O = dUMP + NH3 | feedback regulation | Gallus gallus | |
| dCMP + H2O = dUMP + NH3 | feedback regulation | Tequatrovirus T4 | |
| dCMP + H2O = dUMP + NH3 | feedback regulation | Escherichia phage T2 | |
| dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Gallus gallus | |
| dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Tequatrovirus T4 | |
| dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Escherichia phage T2 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 430 | - |
- |
Escherichia phage T2 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, neutral pH, 0.1 M 2-mercaptoethanol, stable for several months | Escherichia phage T2 |
| frozen in absence of 2-mercaptoethanol, indefinitely stable | Escherichia phage T2 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxymethyl-dCMP + H2O | - |
Gallus gallus | 5-hydroxymethyl-dUMP + NH3 | - |
? | |
| 5-hydroxymethyl-dCMP + H2O | very poor substrate | Tequatrovirus T4 | 5-hydroxymethyl-dUMP + NH3 | - |
? | |
| 5-hydroxymethyl-dCMP + H2O | very poor substrate | Escherichia phage T2 | 5-hydroxymethyl-dUMP + NH3 | - |
? | |
| CMP + H2O | - |
Tequatrovirus T4 | UMP + NH3 | - |
? | |
| CMP + H2O | - |
Escherichia phage T2 | UMP + NH3 | - |
? | |
| dCMP + H2O | - |
Gallus gallus | dUMP + NH3 | - |
? | |
| dCMP + H2O | - |
Tequatrovirus T4 | dUMP + NH3 | - |
? | |
| dCMP + H2O | - |
Escherichia phage T2 | dUMP + NH3 | - |
? | |
| dCMP + H2O | major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway | Gallus gallus | dUMP + NH3 | - |
? | |
| dCMP + H2O | major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway | Tequatrovirus T4 | dUMP + NH3 | - |
? | |
| dCMP + H2O | major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway | Escherichia phage T2 | dUMP + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 identical subunits | Gallus gallus |
| hexamer | 6 identical subunits | Tequatrovirus T4 |
| hexamer | 6 identical subunits | Escherichia phage T2 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Tequatrovirus T4 |
| 37 | - |
assay at | Escherichia phage T2 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
dCTP increases pH-optimum by approximately 3 pH units | Escherichia phage T2 |
| additional information | - |
dCTP increases pH-optimum by approximately 0.6 unit | Gallus gallus |
| 5.3 | - |
in the absence of dCTP | Escherichia phage T2 |
| 8.3 | - |
in the presence of dCTP or 5-hydroxymethyl-dCTP | Escherichia phage T2 |
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