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Literature summary for 3.5.4.12 extracted from
- Deoxycytidylate deaminase. Properties of the enzyme from cultured kidney cells of baby hamster (1974), Biochem. J., 141, 211-217.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dCTP | positive allosteric effector | Cricetinae |  |
| dCTP | maximum activation at 0.04 mM, dCTP activation requires presence of Mg2+ or Mn2+ | Cricetinae | |
General Stability
| General Stability | Organism |
|---|---|
| dCTP and Mg2+ stabilize | Cricetinae |
| dCTP, 0.125 mM, or ethyleneglycol, 20% v/v, stabilize | Cricetinae |
| enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and ethyleneglycol, 20% v/v | Cricetinae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dTTP | allosteric inhibitor; maximum inhibition at 0.015 mM, inhibition requires presence of Mg2+ or Mn2+ | Cricetinae | |
| EDTA | 1 mM: 90% inhibition, restored to maximum by 1-5 mM Mg2+ or Mn2+ | Cricetinae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.91 | - |
dCMP | - |
Cricetinae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for complete activation by dCTP | Cricetinae | |
| Mg2+ | dCTP activation and dTTP inhibition requires presence of Mg2+ or Mn2+ | Cricetinae | |
| Mg2+ | required for activation by dCTP and for inhibition by dTTP | Cricetinae | |
| Mg2+ | required for activation by dCTP, Mg2+ in the absence of dCTP: no effect | Cricetinae | |
| Mn2+ | dCTP activation and dTTP inhibition requires presence of Mg2+ or Mn2+ | Cricetinae | |
| additional information | metal ions not required | Cricetinae | |
| additional information | bound bivalent metal cations probably present | Cricetinae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
BHK-21/C13 cells, sucrose density gradient centrifugation | Cricetinae |
| 130000 | - |
BHK-21/C13 cells, gel filtration | Cricetinae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCMP + H2O | Cricetinae | enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis | dUMP + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetinae | - |
BHK-21/C13 cells, baby-hamster | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, BHK-21/C13 cells, baby-hamster | Cricetinae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Cricetinae | |
| dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Cricetinae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| BHK-21 cell | baby-hamster kidney | Cricetinae | - |
| kidney | BHK-21/C13 cells, baby-hamster kidney cells | Cricetinae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.37 | - |
- |
Cricetinae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C | Cricetinae |
| -70°C, 20% v/v ethyleneglycol, stable for 2 months | Cricetinae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxymethyl-dCMP + H2O | - |
Cricetinae | 5-hydroxymethyl-dUMP + NH3 | - |
? | |
| 5-methyl-dCMP + H2O | - |
Cricetinae | 5-methyl-dUMP + NH3 | - |
? | |
| dCMP + H2O | - |
Cricetinae | dUMP + NH3 | - |
? | |
| dCMP + H2O | enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis | Cricetinae | dUMP + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cricetinae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and ethyleneglycol, 20% v/v | Cricetinae |
| 50 | - |
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and Mg2+, or by increasing protein concentration | Cricetinae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
BHK-21/C13 cells | Cricetinae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7.0: 60%, pH 8.2: 98% and pH 9.0 96% of maximum activity | Cricetinae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no coenzyme required | Cricetinae |
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