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Literature summary for 3.5.4.12 extracted from
- Deoxycytidylate deaminase from T2-infected Escherichia coli (1978), Methods Enzymol., 51, 412-418.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5-hydroxymethyl-dCTP | - |
Escherichia phage T2 |  |
| dCTP | positive allosteric effector | Escherichia phage T2 | |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Escherichia phage T2 |
| dCTP, or to a lesser extent dTTP, stabilize against heat inactivation, protein denaturants and proteolytic enzymes | Escherichia phage T2 |
| thiols stabilize | Escherichia phage T2 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dTTP | allosteric inhibitor | Escherichia phage T2 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic properties and parameters | Escherichia phage T2 | |
| 0.1 | - |
dCMP | pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km | Escherichia phage T2 | |
| 0.1 | - |
5-methyl-dCMP | pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km | Escherichia phage T2 | |
| 10 | - |
CMP | pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km | Escherichia phage T2 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+ | Escherichia phage T2 | |
| divalent cations | reaction completely dependent on divalent cations | Escherichia phage T2 | |
| Mg2+ | reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+ | Escherichia phage T2 | |
| Mn2+ | reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+ | Escherichia phage T2 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 124000 | - |
PAGE and sedimentation equilibrium | Escherichia phage T2 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCMP + H2O | Escherichia phage T2 | true substrate probably dCMP-Mg complex | dUMP + NH3 | - |
? | |
| dCMP + H2O | Escherichia phage T2 | pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP | dUMP + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia phage T2 | - |
T2-phage infected Escherichia coli | - |
| no activity in Escherichia coli | - |
enzyme not detectable in Escherichia coli until after infection with T-even bacteriophage | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| T2-phage infected Escherichia coli | Escherichia phage T2 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Escherichia phage T2 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 430 | - |
- |
Escherichia phage T2 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, neutral pH, 0.1 M 2-mercaptoethanol, stable for several months | Escherichia phage T2 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-bromo-dCMP + H2O | - |
Escherichia phage T2 | 5-bromo-dUMP + NH3 | - |
? | |
| 5-chloro-dCMP + H2O | - |
Escherichia phage T2 | 5-chloro-dUMP + NH3 | - |
? | |
| 5-fluoro-dCMP + H2O | - |
Escherichia phage T2 | 5-fluoro-dUMP + NH3 | - |
? | |
| 5-hydroxymethyl-dCMP + H2O | very poor substrate | Escherichia phage T2 | 5-hydroxymethyl-dUMP + NH3 | - |
? | |
| 5-iodo-dCMP + H2O | very poor substrate | Escherichia phage T2 | 5-iodo-dUMP + NH3 | - |
? | |
| 5-methyl-dCMP + H2O | - |
Escherichia phage T2 | 5-methyl-dUMP + NH3 | - |
? | |
| CMP + H2O | - |
Escherichia phage T2 | UMP + NH3 | - |
? | |
| dCMP + H2O | true substrate probably dCMP-Mg complex | Escherichia phage T2 | dUMP + NH3 | - |
? | |
| dCMP + H2O | pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP | Escherichia phage T2 | dUMP + NH3 | - |
? | |
| additional information | substrate specificity | Escherichia phage T2 | ? | - |
? | |
| additional information | catalyzes deamination of halogenated derivatives of dCMP, but appears to be restricted in its ability to deaminate substrates with a large group in the 5-position | Escherichia phage T2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 identical subunits | Escherichia phage T2 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
dCTP, and to a lesser extent dTTP, stabilizes against heat inactivation | Escherichia phage T2 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
in the absence of dCTP | Escherichia phage T2 |
| 8 | - |
assay at | Escherichia phage T2 |
| 8.3 | - |
in the presence of dCTP or 5-hydroxymethyl-dCTP | Escherichia phage T2 |
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