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Literature summary for 3.5.4.10 extracted from

  • Vergis, J.M.; Bulock, K.G.; Fleming, K.G.; Beardsley, G.P.
    Human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase. A bifunctional protein requiring dimerization for transformylase activity but not for cyclohydrolase activity (2001), J. Biol. Chem., 276, 7727-7733.
    View publication on PubMed
Search for more literature for 3.5.4.10

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D125A dramatically reduced kcat-value Homo sapiens
D125E dramatically reduced kcat-value Homo sapiens
D125N dramatically reduced kcat-value Homo sapiens
K137A dramatically reduced kcat-value Homo sapiens
K137R dramatically reduced kcat-value Homo sapiens
K66A dramatically reduced kcat-value Homo sapiens
Y104A dramatically reduced kcat-value Homo sapiens
Y104A/D125A enzyme activity very low but detectable, contrary to wild type a minimal dependence of specific activity on concentration Homo sapiens
Y104F dramatically reduced kcat-value Homo sapiens
Y104F/D125A enzyme activity very low but detectable, contrary to wild type a minimal dependence of specific activity on concentration Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, wild type Homo sapiens
0.0027
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125N Homo sapiens
0.0027
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137R Homo sapiens
0.003
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant Y104A Homo sapiens
0.0036
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant Y104F Homo sapiens
0.0052
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125E Homo sapiens
0.0091
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125A Homo sapiens
0.031
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137A Homo sapiens
0.115
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K66A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 bifunctional enzyme 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase
-

Reaction

Reaction Comment Organism Reaction ID
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide orbital steering mechanism Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide
-
 Homo sapiens IMP + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125A Homo sapiens
0.036
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant Y104F Homo sapiens
0.062
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K66A Homo sapiens
0.136
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant Y104A Homo sapiens
0.147
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125E Homo sapiens
0.276
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137A Homo sapiens
0.507
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137R Homo sapiens
0.603
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125N Homo sapiens
6.08
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant D125N Homo sapiens
6.08
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, mutant K137R Homo sapiens
8.6
-
 5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide pH 7.5, wild type Homo sapiens