Literature summary for 3.5.4.1 extracted from

Dubeau, M.; Ghinet, M.G.; Jacques, P.; Clermont, N.; Beaulieu, C.; Brzezinski, R.
Cytosine deaminase as a negative selection marker for gene disruption and replacement in the genus Streptomyces and other actinobacteria (2009), Appl. Environ. Microbiol., 75, 1211-1214.

Search for more literature for 3.5.4.1

Application

Application	Comment	Organism
pharmacology	negative selection system for actinobacteria based on cytosine deaminase	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
pMP201 vector and derivatives with aminoglycoside resistance gene, synthetic version of the cytosine deaminase gene (CodA), optimized for expression in Streptomyces species, generation of Streptomyces lividans TK24 protoplasts, transformation of Streptomyces lividans	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D314A	PCR-directed mutagenesis, activity against 5-fluorocytosine 2-fold increased, 17-fold lower sensitivity against native cytosine	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5-fluorocytosine + H2O	Escherichia coli	used as negative selection marker system, increased 5-fluorocytosine sensitivity in Streptomyces lividans	5-fluorouracil + NH3	-	?
cytosine + H2O	Escherichia coli	-	uracil + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P25524	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	cytosine deaminase of Escherichia coli used as a negative selection marker in Streptomyces, can be applied for any actinobacterial species naturally resistant to 5-fluorocytosine while sensitive to 5-fluorouracil, valuable addition to the actinobacterial genetic toolbox	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-fluorocytosine + H2O	used as negative selection marker system, increased 5-fluorocytosine sensitivity in Streptomyces lividans	Escherichia coli	5-fluorouracil + NH3	-	?
cytosine + H2O	-	Escherichia coli	uracil + NH3	-	?

Synonyms

Synonyms	Comment	Organism
CodA protein	-	Escherichia coli

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Release 2023.1 (January 2023)