Literature summary for 3.5.4.1 extracted from

Fogar, P.; Navaglia, F.; Basso, D.; Greco, E.; Zambon, C.F.; Fadi, E.; Falda, A.; Stranges, A.; Vannozzi, F.; Danesi, R.; Pedrazzoli, S.; Plebani, M.
Suicide gene therapy with the yeast fusion gene cytosine deaminase/uracil phosphoribosyltransferase is not enough for pancreatic cancer (2007), Pancreas, 35, 224-231.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression as fusion protein with uracil phosphoribosyltransferase in human pancreatic and colorectal cancer cell lines AsPC1, BxPC3, CaPan1, MIA PaCa2, Panc1, and HT-29, overview	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a fusion protein of yeast cytosine deaminase with yeast uracil phosphoribosyltransferase	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q12178	gene FCY1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-fluorocytosine + H2O	activation of the prodrug to the cytotoxic drug in cancer therapy, gene therapy with the FCY1 gene, encoding the enzyme, alone is not effective in treatement of pancreatic cancer, co-expression with uracil phosphoribosyltranferase encoding gene FUR1, as fusion enzyme, can confer sensitivity to 5-fluorouracil to some pancreatic cancer cells, but not to all, thus it might also not be an effective therapy in pancreatic cancer, overview	Saccharomyces cerevisiae	5-fluorouracil + NH3	-	?

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Release 2023.1 (January 2023)