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Literature summary for 3.5.3.9 extracted from
- An enzyme cycling method for measurement of allantoin in human serum (2008), Anal. Biochem., 378, 65-70.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of an enzyme cycling method for measuring allantoin concentrations in human serum | Metabacillus fastidiosus |
General Stability
| General Stability | Organism |
|---|---|
| preparation of allantoate amidohydrolase stabilized by the addition of 0.1 mM EDTA | Metabacillus fastidiosus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | essential for enzyme activity, catalyzes the second step of the developed assay, ammonia removal reaction in the presence of | Metabacillus fastidiosus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allantoate + H2O | Metabacillus fastidiosus | - |
ureidoglycine + NH3 + CO2 | - |
? | |
| allantoate + H2O | Metabacillus fastidiosus ATCC 29604 | - |
ureidoglycine + NH3 + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metabacillus fastidiosus | - |
ATCC29604 | - |
| Metabacillus fastidiosus ATCC 29604 | - |
ATCC29604 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme cycling method for measuring allantoin concentrations in human serum, ammonia is liberated from allantoate by the activity of allantoate amidohydrolase, 70 microM allantoin and a concentration of 2 U/ml allantoate amidohydrolase required for maximum absorbance, measurement of allantoin levels in healthy subjects | Metabacillus fastidiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allantoate + H2O | - |
Metabacillus fastidiosus | ureidoglycine + NH3 + CO2 | - |
? | |
| allantoate + H2O | - |
Metabacillus fastidiosus ATCC 29604 | ureidoglycine + NH3 + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| allantoate amidohydrolase | - |
Metabacillus fastidiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Metabacillus fastidiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Metabacillus fastidiosus |
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Release 2023.1 (January 2023)
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