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Literature summary for 3.5.3.7 extracted from

  • Saragadam, T.; Kumar, S.; Punekar, N.S.
    Characterization of 4-guanidinobutyrase from Aspergillus niger (2019), Microbiology, 165, 396-410 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Aspergillus niger
EDTA
-
Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
4-Guanidinobutanoate pH 7.5, 37°C Aspergillus niger
53.7
-
3-guanidinopropionate pH 7.5, 37°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ associated with Zn2+ ions Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
336000
-
gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-guanidinobutanoate + H2O Aspergillus niger
-
4-aminobutanoate + urea
-
?
4-guanidinobutanoate + H2O Aspergillus niger NCIM 565
-
4-aminobutanoate + urea
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger NCIM 565
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68
-
pH 7.5, 37°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-guanidinopropionate + H2O the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid Aspergillus niger 3-aminopropionate + urea
-
?
3-guanidinopropionate + H2O the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid Aspergillus niger NCIM 565 3-aminopropionate + urea
-
?
4-guanidinobutanoate + H2O
-
Aspergillus niger 4-aminobutanoate + urea
-
?
4-guanidinobutanoate + H2O the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid Aspergillus niger 4-aminobutanoate + urea
-
?
4-guanidinobutanoate + H2O
-
Aspergillus niger NCIM 565 4-aminobutanoate + urea
-
?
4-guanidinobutanoate + H2O the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid Aspergillus niger NCIM 565 4-aminobutanoate + urea
-
?
additional information other guanidinium compounds, namely L-arginine, agmatine, 2-guanidinoacetic acid, D-arginine and L-homoarginine, are not substrates for this enzyme Aspergillus niger ?
-
?
additional information other guanidinium compounds, namely L-arginine, agmatine, 2-guanidinoacetic acid, D-arginine and L-homoarginine, are not substrates for this enzyme Aspergillus niger NCIM 565 ?
-
?

Subunits

Subunits Comment Organism
homohexamer 6 * 50400, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
GBase
-
Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Aspergillus niger

Expression

Organism Comment Expression
Aspergillus niger the enzyme is strictly inducible by agmatine or 4-guanidinobutanoate up

General Information

General Information Comment Organism
metabolism the enzyme is involved in agmatine catabolism. While 4-guanidinobutanoate is an efficient nitrogen source, Aspergillus niger grows very poorly on 3-guanidinopropionate Aspergillus niger