Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Aspergillus niger | |
EDTA | - |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
4-Guanidinobutanoate | pH 7.5, 37°C | Aspergillus niger | |
53.7 | - |
3-guanidinopropionate | pH 7.5, 37°C | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | associated with Zn2+ ions | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
336000 | - |
gel filtration | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-guanidinobutanoate + H2O | Aspergillus niger | - |
4-aminobutanoate + urea | - |
? | |
4-guanidinobutanoate + H2O | Aspergillus niger NCIM 565 | - |
4-aminobutanoate + urea | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger NCIM 565 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
68 | - |
pH 7.5, 37°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-guanidinopropionate + H2O | the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid | Aspergillus niger | 3-aminopropionate + urea | - |
? | |
3-guanidinopropionate + H2O | the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid | Aspergillus niger NCIM 565 | 3-aminopropionate + urea | - |
? | |
4-guanidinobutanoate + H2O | - |
Aspergillus niger | 4-aminobutanoate + urea | - |
? | |
4-guanidinobutanoate + H2O | the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid | Aspergillus niger | 4-aminobutanoate + urea | - |
? | |
4-guanidinobutanoate + H2O | - |
Aspergillus niger NCIM 565 | 4-aminobutanoate + urea | - |
? | |
4-guanidinobutanoate + H2O | the enzyme acts 25times more efficiently on 4-guanidinobutyrate than on 3-guanidinopropionic acid | Aspergillus niger NCIM 565 | 4-aminobutanoate + urea | - |
? | |
additional information | other guanidinium compounds, namely L-arginine, agmatine, 2-guanidinoacetic acid, D-arginine and L-homoarginine, are not substrates for this enzyme | Aspergillus niger | ? | - |
? | |
additional information | other guanidinium compounds, namely L-arginine, agmatine, 2-guanidinoacetic acid, D-arginine and L-homoarginine, are not substrates for this enzyme | Aspergillus niger NCIM 565 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 50400, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
GBase | - |
Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Aspergillus niger |
Organism | Comment | Expression |
---|---|---|
Aspergillus niger | the enzyme is strictly inducible by agmatine or 4-guanidinobutanoate | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in agmatine catabolism. While 4-guanidinobutanoate is an efficient nitrogen source, Aspergillus niger grows very poorly on 3-guanidinopropionate | Aspergillus niger |