Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Brevibacterium helvolum | |
2-mercaptopropionate | - |
Brevibacterium helvolum | |
3-mercaptoethylamine | - |
Brevibacterium helvolum | |
3-Mercaptopropionate | - |
Brevibacterium helvolum | |
5-Aminovalerate | - |
Brevibacterium helvolum | |
6-aminocaproate | - |
Brevibacterium helvolum | |
agmatine | - |
Brevibacterium helvolum | |
mercaptoethylamine | - |
Brevibacterium helvolum | |
n-Butyrate | - |
Brevibacterium helvolum | |
n-valerate | - |
Brevibacterium helvolum | |
o-phenanthroline | - |
Brevibacterium helvolum | |
thioglycolate | - |
Brevibacterium helvolum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
4-Guanidinobutyrate | - |
Brevibacterium helvolum | |
71 | - |
L-arginine | - |
Brevibacterium helvolum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | firmly bound | Brevibacterium helvolum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, SDS-PAGE | Brevibacterium helvolum |
65000 | - |
2 * 65000, room temperature, SDS-PAGE, compactly associated subunits | Brevibacterium helvolum |
190000 | - |
gel filtration | Brevibacterium helvolum |
194000 | - |
gel filtration | Brevibacterium helvolum |
210000 | - |
gel filtration, undenatured enzyme | Brevibacterium helvolum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Guanidinobutyrate + H2O | Pseudomonas fluorescens | - |
4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | Pimelobacter simplex | - |
4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | Brevibacterium linens | - |
4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | Arthrobacter globiformis | - |
4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | Streptomyces griseus | - |
4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | Brevibacterium helvolum | - |
4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | Corynebacterium ammoniagenes | - |
4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | Brevibacterium lipolyticum | - |
4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | Corynebacterium stationis | - |
4-Aminobutyrate + urea | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
IFO 12137 (ATCC 8010) | - |
Brevibacterium helvolum | - |
IFO 12073 (ATCC 11822) | - |
Brevibacterium linens | - |
IFO 12141 | - |
Brevibacterium lipolyticum | - |
IFO 12678 | - |
Corynebacterium ammoniagenes | - |
IFO 12072 (ATCC 6872) | - |
Corynebacterium stationis | - |
IFO 12144 | - |
Pimelobacter simplex | - |
IFO 12069 (ATCC 6946) | - |
Pseudomonas fluorescens | - |
- |
- |
Streptomyces griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brevibacterium helvolum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
83.8 | - |
- |
Brevibacterium helvolum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Guanidinobutyrate + H2O | - |
Pseudomonas fluorescens | 4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | - |
Pimelobacter simplex | 4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | - |
Brevibacterium linens | 4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | - |
Arthrobacter globiformis | 4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | - |
Streptomyces griseus | 4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | - |
Brevibacterium helvolum | 4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | - |
Corynebacterium ammoniagenes | 4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | - |
Brevibacterium lipolyticum | 4-Aminobutyrate + urea | - |
? | |
4-Guanidinobutyrate + H2O | - |
Corynebacterium stationis | 4-Aminobutyrate + urea | - |
? | |
arginine + H2O | weak activity towards L- and D-arginine | Brevibacterium helvolum | 2,5-diaminopentanoate + urea | - |
? | |
L-canavanine + H2O | - |
Brevibacterium helvolum | L-2-amino-4-(aminooxy)butanoic acid + urea | - |
? | |
additional information | - |
Streptomyces griseus | ? | - |
? | |
additional information | guanidinoacetate, L-guanidinosuccinate, 6-guanidinocaproate, 2-guanidinoethyl sulfate, 4-guanidinobutyramide, taurocyamine, creatine, methylguanidine, N-amidino-L-alanine and agmatine are inert as substrates | Brevibacterium helvolum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 65000, room temperature, SDS-PAGE, compactly associated subunits | Brevibacterium helvolum |
tetramer | 4 * 45000, SDS-PAGE | Brevibacterium helvolum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
residual activity after heating for 60 min 50% of the original activity | Brevibacterium helvolum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Brevibacterium helvolum |