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Literature summary for 3.5.3.6 extracted from

  • Kaur, B.; Kaur, R.
    Purification of a dimeric arginine deiminase from Enterococcus faecium GR7 and study of its anti-cancerous activity (2016), Protein Expr. Purif., 125, 53-60 .
    View publication on PubMed
Search for more literature for 3.5.3.6

Application

Application Comment Organism
drug development possibility of a use of the enzyme as a potential anticancer drug. Purified enzyme exhibits profound antiproliferative activity against Hep-G2 cells. Purified enzyme induces apoptosis in the Hep-G2 cells by DNA fragmentation Enterococcus faecium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94365
-
 MALDI-TOF Enterococcus faecium
95600
-
 non-denaturing PAGE Enterococcus faecium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Enterococcus faecium
-
 L-citrulline + NH3
-
 ?
L-arginine + H2O Enterococcus faecium GR7
-
 L-citrulline + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
-
-
Enterococcus faecium GR7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
16.92fold purification, 49.17% yield Enterococcus faecium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
76.65
-
 pH 5.5, 37°C Enterococcus faecium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Enterococcus faecium L-citrulline + NH3
-
 ?
L-arginine + H2O
-
 Enterococcus faecium GR7 L-citrulline + NH3
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 49100 + 1 * 46500, SDS-PAGE Enterococcus faecium

Synonyms

Synonyms Comment Organism
ADI
-
 Enterococcus faecium