Any feedback?
Literature summary for 3.5.3.6 extracted from
- Crystallization and properties of L-arginine deiminase of Pseudomonas putida (1975), J. Biol. Chem., 250, 4580-4583.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Pseudomonas putida |  |
| Al3+ | - |
Pseudomonas putida | |
| Cu2+ | - |
Pseudomonas putida | |
| Fe2+ | - |
Pseudomonas putida | |
| Fe3+ | - |
Pseudomonas putida | |
| Hg2+ | - |
Pseudomonas putida | |
| L-Alpha-amino-beta-guanidinopropionic acid | competitive | Pseudomonas putida | |
| L-Alpha-amino-gamma-guanidinobutyric acid | competitive | Pseudomonas putida | |
| L-homoarginine | competitive | Pseudomonas putida | |
| PCMB | - |
Pseudomonas putida | |
| Sn2+ | - |
Pseudomonas putida | |
| Zn2+ | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
L-Arg | - |
Pseudomonas putida | |
| 2.38 | - |
L-Alpha-amino-beta-guanidinopropionic acid | - |
Pseudomonas putida | |
| 3.03 | - |
L-Alpha-amino-gamma-guanidinobutyric acid | - |
Pseudomonas putida | |
| 3.38 | - |
L-nitroarginine | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
2 * 54000, SDS-PAGE | Pseudomonas putida |
| 120000 | - |
equilibrium sedimentation | Pseudomonas putida |
| 130000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 58.8 | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 8.0, stable for 1 week | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alpha-amino-beta-guanidinepropionic acid + H2O | - |
Pseudomonas putida | 2-amino-3-carbamoylpropionic acid + NH3 | - |
? | |
| L-alpha-amino-beta-guanidinopropionic acid + H2O | - |
Pseudomonas putida | 2-amino-3-carbamoylpropionic acid + NH3 | - |
? | |
| L-alpha-amino-gamma-guanidinobutyric acid + H2O | - |
Pseudomonas putida | 2-amino-4-carbamoylbutanoic acid + NH3 | - |
? | |
| L-Arg + H2O | - |
Pseudomonas putida | L-citrulline + NH3 | - |
? | |
| L-nitroarginine + H2O | - |
Pseudomonas putida | NH3 + nitrocitrulline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 54000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Pseudomonas putida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 53 | 35°C: about 55% of maximal activity, 53°C: about 50% of maximal activity | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
1 h, stable | Pseudomonas putida |
| 55 | - |
3 min, 50% loss of activity | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | pH 5.0: about 40% of maximal activity, pH 7.5: about 50% of maximal activity | Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
rapid loss of activity | Pseudomonas putida |
| 8 | - |
4°C, stable for 1 week | Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
