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Literature summary for 3.5.3.4 extracted from

  • Vigetti, D.; Monetti, C.; Pollegioni, L.; Taramelli, R.; Bernardini, G.
    Xenopus allantoicase: Molecular cloning, enzymatic activity and developmental expression (2000), Arch. Biochem. Biophys., 379, 90-96.
    View publication on PubMed
Search for more literature for 3.5.3.4

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, more than 200% of initial activity Xenopus laevis
EGTA 1 mM, about 200% of initial activity Xenopus laevis
additional information no activation by EDTA or EGTA Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xenopus laevis
expression in Escherichia coli Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, about 50% of inhibition, reversed by EDTA Xenopus laevis
Mg2+ 1 mM, about 50% of inhibition, reversed by EGTA Xenopus laevis
additional information not inhibitory: Ca2+, Mg2+, EDTA, EGTA Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5
-
 Allantoate pH 7.0, 25°C Xenopus laevis
8.44
-
 Allantoate
-
 Xenopus laevis
8.44
-
 Allantoate from kidney Xenopus laevis
250
-
 Allantoate pH 7.0, 25°C Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43700
-
 x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE Xenopus laevis
44700
-
 x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE Xenopus laevis
46500
-
 x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE Mus musculus
47200
-
 x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9JHX6
-
-
Xenopus laevis
-
-
-
Xenopus laevis Q9W6S5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xenopus laevis
recombinant enzyme Xenopus laevis
recombinant enzyme Mus musculus

Renatured (Commentary)

Renatured (Comment) Organism
inhibition by divalent cations is reversed by excess of chaletors of these cations Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Xenopus laevis
-
liver
-
 Xenopus laevis
-
lung
-
 Xenopus laevis
-

Storage Stability

Storage Stability Organism
-20°C, 50 mM phosphate buffer, pH 7.0, stable Xenopus laevis
-20°C, 50 mM phosphate buffer, pH 7.0, stable Mus musculus
4°C, above, rapid loss of activity Xenopus laevis
4°C, above, rapid loss of activity Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-ureidoglycolate
-
 Xenopus laevis glyoxylate + urea
-
 ?
allantoate + H2O
-
 Xenopus laevis (-)-ureidoglycolate + urea
-
 r
allantoate + H2O
-
 Xenopus laevis (S)-ureidoglycolate + urea
-
 ?
allantoate + H2O
-
 Mus musculus (S)-ureidoglycolate + urea
-
 ?
allantoate + H2O
-
 Xenopus laevis ureidoglycolate + urea
-
 ?

Subunits

Subunits Comment Organism
? x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE Xenopus laevis
? x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
allantoicase
-
 Xenopus laevis
XAlc
-
 Xenopus laevis
XAlc
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Xenopus laevis
50 55
-
 Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min, loss of about 50% of activity Xenopus laevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Xenopus laevis
7
-
 assay at Mus musculus