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Literature summary for 3.5.3.3 extracted from
- High-level production of creatine amidinohydrolase from Arthrobacter nicotianae 23710 in Escherichia coli (2015), Appl. Biochem. Biotechnol., 175, 2564-2573.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Glutamicibacter nicotianae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1% residual activity art1mM | Glutamicibacter nicotianae |  |
| Hg2+ | 1% residual activity at1mM | Glutamicibacter nicotianae | |
| additional information | not inhibited by EDTA and NaN3 | Glutamicibacter nicotianae | |
| SDS | - |
Glutamicibacter nicotianae | |
| Zn2+ | 44% residual activity at 1mM | Glutamicibacter nicotianae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 53.2 | - |
Creatine | at pH 7.5 and 37°C | Glutamicibacter nicotianae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by Fe3+, Ba2+, Mn2+, Mg2+, Li+, and Ca2+ | Glutamicibacter nicotianae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46400 | - |
x * 46400, SDS-PAGE | Glutamicibacter nicotianae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatine + H2O | Glutamicibacter nicotianae | - |
sarcosine + urea | - |
? | |
| creatine + H2O | Glutamicibacter nicotianae 23710 | - |
sarcosine + urea | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Triton X-100 | the recombinant creatinase shows excellent resistance to 1 g/l Triton X-100 | Glutamicibacter nicotianae |
| Tween 20 | the recombinant creatinase shows excellent resistance to 1 g/l Tween 20 | Glutamicibacter nicotianae |
| Tween 80 | the recombinant creatinase shows excellent resistance to 1 g/l Tween 80 | Glutamicibacter nicotianae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glutamicibacter nicotianae | A0A089FQH5 | - |
- |
| Glutamicibacter nicotianae 23710 | A0A089FQH5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, HiPrep column chromatography, and Superdex 200 gel filtration | Glutamicibacter nicotianae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0014 | - |
crude enzyme, at pH 7.5 and 37°C | Glutamicibacter nicotianae |
| 0.0203 | - |
after 14.1fold purification, at pH 7.5 and 37°C | Glutamicibacter nicotianae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatine + H2O | - |
Glutamicibacter nicotianae | sarcosine + urea | - |
? | |
| creatine + H2O | - |
Glutamicibacter nicotianae 23710 | sarcosine + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46400, SDS-PAGE | Glutamicibacter nicotianae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Creatine amidinohydrolase | - |
Glutamicibacter nicotianae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
the temperature optimum is at about 37°C, while the enzymatic activity sharply decreases above 40°C | Glutamicibacter nicotianae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | about 65% activity at 25°C, about 77% activity at 30°C, about 85% activity at 35°C, 100% activity at 37°C, about 95% activity at 40°C, about 70% activity at 45°C, about 20% activity at 50°C | Glutamicibacter nicotianae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | the enzyme is stable below 40°C but completely loses its activity after 30 min preincubation at 50°C | Glutamicibacter nicotianae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Glutamicibacter nicotianae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | 60% activity at pH 6.5 and 8.0 | Glutamicibacter nicotianae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | the enzyme is stable in the range of pH 6.0 to 11.0 for 6 h incubation | Glutamicibacter nicotianae |
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