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Literature summary for 3.5.3.3 extracted from
- Study on the creatinase from Paracoccus sp. strain WB1 (2006), Process Biochem., 41, 2072-2077.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic tree, overview | Paracoccus sp. WB1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inactivation at 1 mM | Paracoccus sp. WB1 |  |
| Co2+ | 50% inhibition at 1 mM | Paracoccus sp. WB1 | |
| Cu2+ | complete inactivation at 1 mM | Paracoccus sp. WB1 | |
| Hg2+ | complete inactivation at 1 mM | Paracoccus sp. WB1 | |
| additional information | NaN3, and chelating agents EDTA and 1,10-phenanthroline do not inhibit the activity at the concentration of 20 mM, no inhibition by Tween80 and SDS, and by Ca2+, Pb2+, Ba2+, Fe2+, Fe3+, Mg2+, Cr2+, Mn2+, and Li+ | Paracoccus sp. WB1 | |
| Tween20 | strong inhibition at 1 mM | Paracoccus sp. WB1 | |
| Zn2+ | 40% inhibition at 1 mM | Paracoccus sp. WB1 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
Creatine | pH 7.5, 37°C | Paracoccus sp. WB1 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, SDS-PAGE | Paracoccus sp. WB1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatine + H2O | Paracoccus sp. WB1 | - |
sarcosine + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus sp. WB1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 10.17fold to homogeneity by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration | Paracoccus sp. WB1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
measurement of creatinine in a coupled assay with creatininase and sarcosine oxidase, overview | Paracoccus sp. WB1 |
| 6.1 | - |
purified enzyme | Paracoccus sp. WB1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatine + H2O | - |
Paracoccus sp. WB1 | sarcosine + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE | Paracoccus sp. WB1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Paracoccus sp. WB1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | - |
Paracoccus sp. WB1 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | purified enzyme, stable | Paracoccus sp. WB1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Paracoccus sp. WB1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | - |
Paracoccus sp. WB1 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | purified enzyme, stable | Paracoccus sp. WB1 |
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