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Literature summary for 3.5.3.26 extracted from

  • Shin, I.; Percudani, R.; Rhee, S.
    Structural and functional insights into (S)-ureidoglycine aminohydrolase, key enzyme of purine catabolism in Arabidopsis thaliana (2012), J. Biol. Chem., 287, 18796-18805.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.26

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834 and BL21 (DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
seleno-L-methionine-labelled enzyme, hanging drop vapor diffusion method, using either 0.1 M phosphate citrate, pH 4.2, 10% (w/v) PEG 3000, 0.2 M NaCl or 0.1 M HEPES, pH 7.5, 7% (w/v) PEG 8000, 8% (v/v) ethylene glycol Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
E235A inactive Arabidopsis thaliana
E235Q inactive Arabidopsis thaliana
H237A inactive Arabidopsis thaliana
H241A inactive Arabidopsis thaliana
K291A inactive Arabidopsis thaliana
K291R the mutant shows reduced activity compared to the wild type Arabidopsis thaliana
Q275A inactive Arabidopsis thaliana
Y252F the mutant shows reduced activity compared to the wild type Arabidopsis thaliana
Y287A inactive Arabidopsis thaliana
Y287F inactive Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.77
-
 (S)-2-ureidoglycine wild type enzyme, at pH 8.5 and 30°C Arabidopsis thaliana
2.3
-
 (S)-2-ureidoglycine mutant enzyme Y252F, at pH 8.5 and 30°C Arabidopsis thaliana
3.46
-
 (S)-2-ureidoglycine mutant enzyme K291R, at pH 8.5 and 30°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ contains Mn2+, the Mn2+ ion acts as a molecular anchor to bind (S)-ureidoglycine, and its binding mode dictates the enantioselectivity of the reaction Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30200
-
 8 * 30200, SDS-PAGE Arabidopsis thaliana
200000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-2-ureidoglycine + H2O Arabidopsis thaliana
-
 (S)-ureidoglycolate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8GXV5
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity column chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-ureidoglycine + H2O
-
 Arabidopsis thaliana (S)-ureidoglycolate + NH3
-
 ?

Subunits

Subunits Comment Organism
homooctamer 8 * 30200, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
UGLYAH
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
73
-
 (S)-2-ureidoglycine mutant enzyme Y252F, at pH 8.5 and 30°C Arabidopsis thaliana
493
-
 (S)-2-ureidoglycine mutant enzyme K291R, at pH 8.5 and 30°C Arabidopsis thaliana
761
-
 (S)-2-ureidoglycine wild type enzyme, at pH 8.5 and 30°C Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
32
-
 (S)-2-ureidoglycine mutant enzyme Y252F, at pH 8.5 and 30°C Arabidopsis thaliana
143
-
 (S)-2-ureidoglycine mutant enzyme K291R, at pH 8.5 and 30°C Arabidopsis thaliana
429
-
 (S)-2-ureidoglycine wild type enzyme, at pH 8.5 and 30°C Arabidopsis thaliana