Literature summary for 3.5.3.22 extracted from

Shrestha, B.; Dhakal, D.; Darsandhari, S.; Pandey, R.; Pokhrel, A.; Jnawali, H.; Sohng, J.
Heterologous production of clavulanic acid intermediates in Streptomyces venezuelae (2017), Biotechnol. Bioprocess Eng., 22, 359-365 .

No PubMed abstract available

Application

Application	Comment	Organism
synthesis	clavulanic acid intermediates: deoxygaunidinoproclavaminic acid, guanidinoproclavaminic acid, and dihydroclavaminic acid are heterologously produced in Streptomyces venezuelae recombinant using four sets of early genes from the clavulanic acid biosynthetic pathway	Streptomyces clavuligerus
synthesis	expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (Ceas2), beta-lactam synthetase (Bls2), clavaminate synthase (Cas2), and proclavaminate amidinohydrolase (Pah2), in Streptomyces venezuelae enables production of clavulanic acid intermediates deoxygaunidinoproclavaminic acid, guanidinoproclavaminic acid, and dihydroclavaminic acid	Streptomyces clavuligerus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Streptomyces venezuelae	Streptomyces clavuligerus
Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028	Streptomyces clavuligerus

Cloned (Comment)

Organism

expression in Streptomyces venezuelae

Streptomyces clavuligerus

Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028

Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
guanidinoproclavaminic acid + H2O	Streptomyces clavuligerus	the enzyme is involved in clavulanic acid biosynthesis	proclavaminic acid + urea	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces clavuligerus	B5GLC8	-	-
Streptomyces clavuligerus	P0DJQ3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
guanidinoproclavaminic acid + H2O	the enzyme is involved in clavulanic acid biosynthesis	Streptomyces clavuligerus	proclavaminic acid + urea	-	?

Synonyms

Synonyms	Comment	Organism
PAH2	-	Streptomyces clavuligerus

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in clavulanic acid biosynthesis	Streptomyces clavuligerus