BRENDA - Enzyme Database
show all sequences of 3.5.3.20

Diguanidinobutanase of Pseudomonas putida and enzymatic preparation of agmatine from 1,4-diguanidinobutanate

Shoeb, S.M.; Kaneoke, M.; Shimizu, E.; Yorifuji, T.; Appl. Microbiol. Biotechnol. 44, 94-99 (1995)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
preparation of pure agmatine sulfate
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-Diaminodecane
41% inhibition at concentration of 3.0 mM
Pseudomonas putida
1,7-Diaminoheptane
22% inhibition at concentration of 5.0 mM
Pseudomonas putida
1,8-diaminooctane
34% inhibition at concentration of 3.0 mM
Pseudomonas putida
1,9-diaminononane
38% inhibition at concentration of 3.0 mM
Pseudomonas putida
1-amino-5-guanidinopentane
29% inhibition at concentration of 5.0 mM
Pseudomonas putida
5-Guanidinovalerate
10% inhibition at concentration of 5.0 mM
Pseudomonas putida
agmatine
28% inhibition at concentration of 5.0 mM
Pseudomonas putida
DTNB
11% inhibition at concentration of 5.0 mM
Pseudomonas putida
N-carbamoylagmatine
62% inhibition at concentration of 5.0 mM
Pseudomonas putida
p-chloromercuribenzoate
complete loss of activity at concentration of 5.0 mM, 30% reactivation by incubation with 10.0 mM 2-mercaptoethanol
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
1,8-Diguanidinooctane
-
Pseudomonas putida
0.24
-
1,7-Diguanidinoheptane
-
Pseudomonas putida
0.33
-
1,6-Diguanidinohexane
-
Pseudomonas putida
0.5
-
1,5-Diguanidinopentane
-
Pseudomonas putida
0.65
-
1,4-Diguanidinobutane
-
Pseudomonas putida
0.91
-
1,3-diguanidinopropane
-
Pseudomonas putida
1
-
1-amino-7-guanidinoheptane
-
Pseudomonas putida
1.3
-
1-amino-6-guanidinohexane
-
Pseudomonas putida
2.5
-
1-amino-5-guanidinopentane
-
Pseudomonas putida
3
-
agmatine
-
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
inert
Pseudomonas putida
Co2+
required for full activity
Pseudomonas putida
Mg2+
inert
Pseudomonas putida
Mn2+
required for full activity, no inactivation by incubation with 10 mM EDTA; two ions per subunit
Pseudomonas putida
Ni2+
required for full activity
Pseudomonas putida
Zn2+
inert
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
170000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,4-diguanidinobutane + H2O
Pseudomonas putida
-
agmatine + urea
-
Pseudomonas putida
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
full purification
Pseudomonas putida
Storage Stability
Storage Stability
Organism
pH 7.0-8.0
Pseudomonas putida
pH 7.0-9.0
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-diguanidinobutane + H2O
-
209444
Pseudomonas putida
agmatine + urea
-
209444
Pseudomonas putida
?
1-amino-5-guanidinopentane + H2O
-
209444
Pseudomonas putida
1,5-diaminopentane + urea
-
-
-
?
1-amino-6-guanidinohexane + H2O
-
209444
Pseudomonas putida
1,6-diaminohexane + urea
-
-
-
?
1-amino-7-guanidinoheptane + H2O
-
209444
Pseudomonas putida
1,7-diaminoheptane + urea
-
-
-
?
agmatine + H2O
low activity towards agmatine
209444
Pseudomonas putida
putrescine + urea
-
-
-
?
diguanidinoalkanoate + H2O
utilization of alkanoates with 3-10 methylen groups
209444
Pseudomonas putida
aminoalkylguanidine + urea
-
209444
Pseudomonas putida
?
Subunits
Subunits
Commentary
Organism
tetramer
4*38000 Da, SDS-PAGE
Pseudomonas putida
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
no loss of activity within 2 h in presence of 10 mM EDTA
Pseudomonas putida
40
-
stable on heating in presence of Mn2+
Pseudomonas putida
60
-
complete inactivation in presence and absence of Mn2+
Pseudomonas putida
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1330
-
1,4-Diguanidinobutane
per subunit
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
11
in sodium carbonate and piperazine hydrochloric buffers
Pseudomonas putida
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
8
stable in potassium phosphate
Pseudomonas putida
7
9
stable in TRIS-HCl
Pseudomonas putida
10
-
stable in piperazine hydrochloride
Pseudomonas putida
Application (protein specific)
Application
Commentary
Organism
synthesis
preparation of pure agmatine sulfate
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-Diaminodecane
41% inhibition at concentration of 3.0 mM
Pseudomonas putida
1,7-Diaminoheptane
22% inhibition at concentration of 5.0 mM
Pseudomonas putida
1,8-diaminooctane
34% inhibition at concentration of 3.0 mM
Pseudomonas putida
1,9-diaminononane
38% inhibition at concentration of 3.0 mM
Pseudomonas putida
1-amino-5-guanidinopentane
29% inhibition at concentration of 5.0 mM
Pseudomonas putida
5-Guanidinovalerate
10% inhibition at concentration of 5.0 mM
Pseudomonas putida
agmatine
28% inhibition at concentration of 5.0 mM
Pseudomonas putida
DTNB
11% inhibition at concentration of 5.0 mM
Pseudomonas putida
N-carbamoylagmatine
62% inhibition at concentration of 5.0 mM
Pseudomonas putida
p-chloromercuribenzoate
complete loss of activity at concentration of 5.0 mM, 30% reactivation by incubation with 10.0 mM 2-mercaptoethanol
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
1,8-Diguanidinooctane
-
Pseudomonas putida
0.24
-
1,7-Diguanidinoheptane
-
Pseudomonas putida
0.33
-
1,6-Diguanidinohexane
-
Pseudomonas putida
0.5
-
1,5-Diguanidinopentane
-
Pseudomonas putida
0.65
-
1,4-Diguanidinobutane
-
Pseudomonas putida
0.91
-
1,3-diguanidinopropane
-
Pseudomonas putida
1
-
1-amino-7-guanidinoheptane
-
Pseudomonas putida
1.3
-
1-amino-6-guanidinohexane
-
Pseudomonas putida
2.5
-
1-amino-5-guanidinopentane
-
Pseudomonas putida
3
-
agmatine
-
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
inert
Pseudomonas putida
Co2+
required for full activity
Pseudomonas putida
Mg2+
inert
Pseudomonas putida
Mn2+
required for full activity, no inactivation by incubation with 10 mM EDTA; two ions per subunit
Pseudomonas putida
Ni2+
required for full activity
Pseudomonas putida
Zn2+
inert
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
170000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,4-diguanidinobutane + H2O
Pseudomonas putida
-
agmatine + urea
-
Pseudomonas putida
?
Purification (Commentary) (protein specific)
Commentary
Organism
full purification
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
pH 7.0-8.0
Pseudomonas putida
pH 7.0-9.0
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-diguanidinobutane + H2O
-
209444
Pseudomonas putida
agmatine + urea
-
209444
Pseudomonas putida
?
1-amino-5-guanidinopentane + H2O
-
209444
Pseudomonas putida
1,5-diaminopentane + urea
-
-
-
?
1-amino-6-guanidinohexane + H2O
-
209444
Pseudomonas putida
1,6-diaminohexane + urea
-
-
-
?
1-amino-7-guanidinoheptane + H2O
-
209444
Pseudomonas putida
1,7-diaminoheptane + urea
-
-
-
?
agmatine + H2O
low activity towards agmatine
209444
Pseudomonas putida
putrescine + urea
-
-
-
?
diguanidinoalkanoate + H2O
utilization of alkanoates with 3-10 methylen groups
209444
Pseudomonas putida
aminoalkylguanidine + urea
-
209444
Pseudomonas putida
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4*38000 Da, SDS-PAGE
Pseudomonas putida
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
no loss of activity within 2 h in presence of 10 mM EDTA
Pseudomonas putida
40
-
stable on heating in presence of Mn2+
Pseudomonas putida
60
-
complete inactivation in presence and absence of Mn2+
Pseudomonas putida
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1330
-
1,4-Diguanidinobutane
per subunit
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
11
in sodium carbonate and piperazine hydrochloric buffers
Pseudomonas putida
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
8
stable in potassium phosphate
Pseudomonas putida
7
9
stable in TRIS-HCl
Pseudomonas putida
10
-
stable in piperazine hydrochloride
Pseudomonas putida
Other publictions for EC 3.5.3.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
209444
Shoeb
-
Diguanidinobutanase of Pseudom ...
Pseudomonas putida
Appl. Microbiol. Biotechnol.
44
94-99
1995
-
1
-
-
-
-
10
10
-
6
1
1
-
1
-
-
1
-
-
-
-
2
6
1
-
-
3
1
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
10
-
10
-
6
1
1
-
-
-
1
-
-
-
2
6
1
-
-
3
1
1
-
3
-
-
-
-
-
-
-
209443
Yorifuji
-
Degradation of alpha,omega-dig ...
Pseudomonas putida
Agric. Biol. Chem.
53
3003-3009
1989
-
-
-
-
-
-
-
1
-
3
-
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
3
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-