BRENDA - Enzyme Database
show all sequences of 3.5.3.20

Degradation of alpha,omega-diguanidinoalkanoates and a novel enzyme, diguanidinobutane amidohydrolase in Pseudomonas putida

Yorifuji, T.; Kaneoke, M.; Shimizu, E.; Shiota, K.; Matsuo, R.; Agric. Biol. Chem. 53, 3003-3009 (1989)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
1,4-Diguanidinobutane
-
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
required for full activity
Pseudomonas putida
Mn2+
required for full activity, no inactivation by incubation with 10 mM EDTA
Pseudomonas putida
Ni2+
required for full activity
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,4-diguanidinobutane + H2O
Pseudomonas putida
-
agmatine + urea
-
Pseudomonas putida
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
partial purification
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-diguanidinobutane + H2O
-
209443
Pseudomonas putida
agmatine + urea
-
209443
Pseudomonas putida
?
agmatine + H2O
low activity towards agmatine
209443
Pseudomonas putida
putrescine + urea
-
209443
Pseudomonas putida
?
diguanidinoalkanoate + H2O
utilization of alkanoates with 3-10 methylen groups
209443
Pseudomonas putida
aminoalkylguanidine + urea
-
209443
Pseudomonas putida
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable on heating for 15 min
Pseudomonas putida
50
-
unstable on heating for 15 min
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
11
-
Pseudomonas putida
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
10
most stable
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
1,4-Diguanidinobutane
-
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
required for full activity
Pseudomonas putida
Mn2+
required for full activity, no inactivation by incubation with 10 mM EDTA
Pseudomonas putida
Ni2+
required for full activity
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,4-diguanidinobutane + H2O
Pseudomonas putida
-
agmatine + urea
-
Pseudomonas putida
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-diguanidinobutane + H2O
-
209443
Pseudomonas putida
agmatine + urea
-
209443
Pseudomonas putida
?
agmatine + H2O
low activity towards agmatine
209443
Pseudomonas putida
putrescine + urea
-
209443
Pseudomonas putida
?
diguanidinoalkanoate + H2O
utilization of alkanoates with 3-10 methylen groups
209443
Pseudomonas putida
aminoalkylguanidine + urea
-
209443
Pseudomonas putida
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable on heating for 15 min
Pseudomonas putida
50
-
unstable on heating for 15 min
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
11
-
Pseudomonas putida
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
10
most stable
Pseudomonas putida
Other publictions for EC 3.5.3.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
209444
Shoeb
-
Diguanidinobutanase of Pseudom ...
Pseudomonas putida
Appl. Microbiol. Biotechnol.
44
94-99
1995
-
1
-
-
-
-
10
10
-
6
1
1
-
1
-
-
1
-
-
-
-
2
6
1
-
-
3
1
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
10
-
10
-
6
1
1
-
-
-
1
-
-
-
2
6
1
-
-
3
1
1
-
3
-
-
-
-
-
-
-
209443
Yorifuji
-
Degradation of alpha,omega-dig ...
Pseudomonas putida
Agric. Biol. Chem.
53
3003-3009
1989
-
-
-
-
-
-
-
1
-
3
-
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
3
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-