Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | together with Mn2+ | Pseudomonas sp. | |
4-aminobutyrate | 5 mM, relative activity 106% | Pseudomonas sp. | |
4-Guanidinobutyrate | 5 mM, relative activity 109% | Pseudomonas sp. | |
5,5'-dithiobis(2-nitrobenzoate) | 0.1 mM, relative activity 138% | Pseudomonas sp. | |
dithiothreitol | - |
Pseudomonas sp. | |
N-ethylmaleimide | 0.1 mM, relative activity 117% | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
colorless needles | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 1.0 mM 50% inhibition | Pseudomonas sp. | |
Co2+ | 1 mM, plus 0.1 mM Mn2+ complete inhibition | Pseudomonas sp. | |
Cu2+ | 1 mM plus 0.1 mM Mn2+ complete inhibition | Pseudomonas sp. | |
EDTA | complete loss of activity, fully restored with Mn2+ | Pseudomonas sp. | |
Fe2+ | 1 mM, plus 0.1 mM Mn2+ partial inhibition, relative activity 40% | Pseudomonas sp. | |
Ni2+ | 1 mM, plus 0.1 mM Mn2+ complete inhibition | Pseudomonas sp. | |
p-chloromercuribenzoate | - |
Flavobacterium sp. | |
p-chloromercuribenzoate | - |
Pseudomonas sp. | |
Zn2+ | 1 mM, plus 0.1 mM Mn2+ complete inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
Guanidinoacetate | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | CaCl2 1 mM in addition to 0.1 mM MnCl2, relative activity 134% | Pseudomonas sp. | |
Co2+ | - |
Flavobacterium sp. | |
Co2+ | 0.1 mM slight activation | Pseudomonas sp. | |
Fe2+ | 0.1 mM slight activation | Pseudomonas sp. | |
Mg2+ | MgSO4 1 mM in addition to 0.1 mM MnCl2, relative activity 127% | Pseudomonas sp. | |
Mn2+ | dependent, most potent activator | Pseudomonas sp. | |
Ni2+ | 0.1 mM slight activation | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, SDS-PAGE | Pseudomonas sp. |
160000 | - |
gel filtration, BioGel P-200 | Pseudomonas sp. |
163000 | - |
gel filtration | Pseudomonas sp. |
165000 | - |
gel filtration, disc gel electrophoreisis | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanidinoacetate + H2O | Pseudomonas sp. | - |
glycine + urea | - |
? | |
guanidinoacetate + H2O | Pseudomonas putida | - |
glycine + urea | - |
? | |
guanidinoacetate + H2O | Penicillium roqueforti | - |
glycine + urea | - |
? | |
guanidinoacetate + H2O | Flavobacterium sp. | - |
glycine + urea | - |
? | |
guanidinoacetate + H2O | Pseudomonas sp. ATCC 14676 | - |
glycine + urea | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Penicillium roqueforti | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. ATCC 14676 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63.7 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-15°C, 20 mM Tris-HCl buffer, pH 8, 1.0 mM 2-mercaptoethanol, 0.1 mM MnCl2, 6months without loss of activity | Pseudomonas sp. |
4°C, 10 mM Tris-HCl buffer, pH 8, 10 mM 2-mercaptoethanol, 1.0 mM MnCl2, 70% saturated ammonium sulfate, crystalline enzyme can be stored for at least 2 months without serious loss in activity, without added Mn2+ or 2-mercaptoethanol activity rapidly lost with a half-life of about 3 days | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanidinoacetate + H2O | - |
Pseudomonas sp. | glycine + urea | - |
? | |
guanidinoacetate + H2O | - |
Pseudomonas putida | glycine + urea | - |
? | |
guanidinoacetate + H2O | - |
Penicillium roqueforti | glycine + urea | - |
? | |
guanidinoacetate + H2O | - |
Flavobacterium sp. | glycine + urea | - |
? | |
guanidinoacetate + H2O | - |
Pseudomonas sp. ATCC 14676 | glycine + urea | - |
? | |
additional information | - |
Flavobacterium sp. | ? | - |
? | |
additional information | beta-guanidinopropionate, gamma-guanidinobutyrate, creatine, L-arginine and agmatine are no substrates | Pseudomonas sp. | ? | - |
? | |
additional information | beta-guanidinopropionate, gamma-guanidinobutyrate, creatine, L-arginine and agmatine are no substrates | Pseudomonas sp. ATCC 14676 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Flavobacterium sp. |
tetramer | 4 * 38000, SDS-PAGE | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54.1 | - |
Guanidinoacetate | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
ATP | - |
Pseudomonas sp. |