Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.3.18 extracted from

  • Wang, Y.; Hu, S.; Fast, W.
    A click chemistry mediated in vivo activity probe for dimethylarginine dimethylaminohydrolase (2009), J. Am. Chem. Soc., 131, 15096-15097.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis use of inhibitor N-(but-3-yn-1-yl)-2-chloroethanimidamide as a broad-specificity probe for labeling endogenous DDAH isoforms and enzymes with similar pharmacophores. Inhibitor labels the active fraction of DDAH-1 in intact mammalian cells and can be blocked by the presence of competitive reversible and irreversible inhibitors. Incorporation of the alkyne tag allows to derivatize with a variety of reagents after in vivo tagging Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
isoform DDAH-1 bearing an N-terminal Myc-tag, expression in HEK 293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C274A inactive and is not labeled by inhibitor N-(but-3-yn-1-yl)-2-chloroethanimidamide Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N-(but-3-yn-1-yl)-2-chloroethanimidamide click chemistry mediated in vivo activity probe that labels the active fraction of DDAH-1 in intact mammalian cells and that can be blocked by the presence of competitive reversible and irreversible inhibitors Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.35
-
pH not specified in the publication, temperature not specified in the publication Homo sapiens N-(but-3-yn-1-yl)-2-chloroethanimidamide