Application | Comment | Organism |
---|---|---|
analysis | use of inhibitor N-(but-3-yn-1-yl)-2-chloroethanimidamide as a broad-specificity probe for labeling endogenous DDAH isoforms and enzymes with similar pharmacophores. Inhibitor labels the active fraction of DDAH-1 in intact mammalian cells and can be blocked by the presence of competitive reversible and irreversible inhibitors. Incorporation of the alkyne tag allows to derivatize with a variety of reagents after in vivo tagging | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
isoform DDAH-1 bearing an N-terminal Myc-tag, expression in HEK 293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C274A | inactive and is not labeled by inhibitor N-(but-3-yn-1-yl)-2-chloroethanimidamide | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(but-3-yn-1-yl)-2-chloroethanimidamide | click chemistry mediated in vivo activity probe that labels the active fraction of DDAH-1 in intact mammalian cells and that can be blocked by the presence of competitive reversible and irreversible inhibitors | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.35 | - |
pH not specified in the publication, temperature not specified in the publication | Homo sapiens | N-(but-3-yn-1-yl)-2-chloroethanimidamide |