Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21star cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxy-nonenal | dose-dependently inhibits DDAH activity with 15% inhibition at 0.01 mM and complete inhibition at 0.5 mM | Homo sapiens | |
H2O2 | 43% inhibition at 1 mM | Homo sapiens | |
additional information | exposure to pathophysiological levels of reactive oxygen has little to no effect on the activity of the enzyme | Homo sapiens | |
OH radical | 47% inhibition at 1 mM | Homo sapiens | |
peroxynitrite | inhibition of 15% after exposure to peroxynitrite at concentrations ranging from 0.01-0.1 mM, 25% inhibition at 1 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.536 | - |
NG-methyl-L-arginine | at 37°C | Homo sapiens | |
0.687 | - |
NG,NG-dimethyl-L-arginine | at 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Zn2+-containing enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
DDAH-1, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and HiTrap Q Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | incapable of hydrolyzing peptide incorporated methylarginines | Homo sapiens | ? | - |
? | |
NG,NG-dimethyl-L-arginine + H2O | - |
Homo sapiens | dimethylamine + L-citrulline | - |
? | |
NG-methyl arginine + H2O | - |
Homo sapiens | L-citrulline + methylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DDAH-1 | - |
Homo sapiens |
dimethylarginine dimethylamino-hydrolase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.05 | - |
at 37°C | Homo sapiens | 4-hydroxy-nonenal |