Cloned (Comment) | Organism |
---|---|
cloned into a pET-28a expression vector, N-terminal His6-tagged protein overexpressed in Escherichia coli BL21 (DE3) | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C249S | Cys249 as the catalytic nucleophile required for intermediate formation | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.143 | - |
S-methyl-L-thiocitrulline | - |
Pseudomonas aeruginosa | |
0.31 | - |
NG,NG-dimethyl-L-arginine | - |
Pseudomonas aeruginosa | |
0.67 | - |
NG-methyl-L-arginine | - |
Pseudomonas aeruginosa | |
1.11 | - |
NG-Amino-L-arginine | - |
Pseudomonas aeruginosa | |
2.3 | - |
NG-Hydroxy-L-arginine | - |
Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
by ESI-MS | Pseudomonas aeruginosa |
30500 | - |
by sequence analysis | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I4E3 | - |
- |
Purification (Comment) | Organism |
---|---|
wild type to more than 98% homogeneity, by Ni-NTA affinity chromatography, mutant required additional purification step, ion-exchange chromatography followed by affinity chromatography, mutant purified to more than 95% homogeneity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
additional information | bad substrates are creatine, creatinine, argininosuccinate, guanidinoacetate, L-homoarginine, L-canvanine | Pseudomonas aeruginosa | ? | - |
? | |
NG,NG-dimethyl-L-arginine + H2O | - |
Pseudomonas aeruginosa | dimethylamine + L-citrulline | - |
? | |
NG-amino-L-arginine + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
NG-hydroxy-L-arginine + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
NG-methyl-L-arginine + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
S-methyl-L-thiocitrulline + H2O | - |
Pseudomonas aeruginosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DDAH | - |
Pseudomonas aeruginosa |
dimethylarginine dimethylaminohydrolase | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
NG-Amino-L-arginine | - |
Pseudomonas aeruginosa | |
0.31 | - |
NG-methyl-L-arginine | - |
Pseudomonas aeruginosa | |
0.33 | - |
NG-Hydroxy-L-arginine | - |
Pseudomonas aeruginosa | |
0.56 | - |
NG,NG-dimethyl-L-arginine | - |
Pseudomonas aeruginosa | |
0.84 | - |
S-methyl-L-thiocitrulline | - |
Pseudomonas aeruginosa |