Application | Comment | Organism |
---|---|---|
medicine | 2-chloroacetamidine may potentially find wide applicability as a general pharmacophore, useful in delineating characteristics of the amidinotransferase superfamily | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C249S | active-site Cys249 residue | Pseudomonas aeruginosa |
H162G | active-site His162 residue | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-chloroacetamidine | irreversible inhibition in a time- and concentration-dependent manner | Pseudomonas aeruginosa | |
additional information | not inhibited by 2-chloroacetamide | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
calculated from the protein sequence of wild-type DDAH, after removal of the N-terminal methionine residue | Pseudomonas aeruginosa |
30500 | - |
deconvoluted mass spectrum of a control reaction mixture that included wild-type DDAH but no inactivator | Pseudomonas aeruginosa |
30560 | - |
fully inactivated wild-type DDAH by 2-chloroacetamidine | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I4E3 | - |
- |
Purification (Comment) | Organism |
---|---|
to more than 95% homogeneity | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
DDAH | - |
Pseudomonas aeruginosa |
dimethylarginine dimethylaminohydrolase | - |
Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
2-chloroacetamidine | - |
Pseudomonas aeruginosa |