BRENDA - Enzyme Database
show all sequences of 3.5.3.16

A new amidinohydrolase, methylguanidine amidinohydrolase from Alcaligenes sp. N-42

Nakajima, M.; Shirokane, Y.; Mizusawa, K.; FEBS Lett. 110, 43-46 (1980)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
useful as a means of detoxication and/or assay of methylguanidine in body fluids
Alcaligenes sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylguanidine + H2O
Alcaligenes sp.
-
methylamine + urea
-
-
?
methylguanidine + H2O
Alcaligenes sp. N-42
-
methylamine + urea
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Alcaligenes sp.
-
N-42
-
Alcaligenes sp. N-42
-
N-42
-
Purification (Commentary)
Commentary
Organism
-
Alcaligenes sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
102
-
-
Alcaligenes sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
agmatine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
?
-
-
-
?
ethylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
ethylamine + urea
-
-
-
?
ethylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
ethylamine + urea
-
-
-
?
guanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
NH3 + urea
-
-
-
?
methylguanidine + H2O
-
209433
Alcaligenes sp.
methylamine + urea
-
-
-
?
methylguanidine + H2O
-
209433
Alcaligenes sp. N-42
methylamine + urea
-
-
-
?
additional information
no hydrolysis of guanidino compounds with carboxyl group
209433
Alcaligenes sp.
?
-
-
-
-
additional information
no hydrolysis of guanidino compounds with carboxyl group
209433
Alcaligenes sp. N-42
?
-
-
-
-
n-butylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
n-butylamine + urea
-
-
-
?
n-butylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
n-butylamine + urea
-
-
-
?
n-propylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
n-propylamine + urea
-
-
-
?
n-propylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
n-propylamine + urea
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
medicine
useful as a means of detoxication and/or assay of methylguanidine in body fluids
Alcaligenes sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylguanidine + H2O
Alcaligenes sp.
-
methylamine + urea
-
-
?
methylguanidine + H2O
Alcaligenes sp. N-42
-
methylamine + urea
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Alcaligenes sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
102
-
-
Alcaligenes sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
agmatine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
?
-
-
-
?
ethylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
ethylamine + urea
-
-
-
?
ethylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
ethylamine + urea
-
-
-
?
guanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
NH3 + urea
-
-
-
?
methylguanidine + H2O
-
209433
Alcaligenes sp.
methylamine + urea
-
-
-
?
methylguanidine + H2O
-
209433
Alcaligenes sp. N-42
methylamine + urea
-
-
-
?
additional information
no hydrolysis of guanidino compounds with carboxyl group
209433
Alcaligenes sp.
?
-
-
-
-
additional information
no hydrolysis of guanidino compounds with carboxyl group
209433
Alcaligenes sp. N-42
?
-
-
-
-
n-butylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
n-butylamine + urea
-
-
-
?
n-butylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
n-butylamine + urea
-
-
-
?
n-propylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp.
n-propylamine + urea
-
-
-
?
n-propylguanidine + H2O
slight hydrolysis, 1.3% of the natural substrate methylguanidine
209433
Alcaligenes sp. N-42
n-propylamine + urea
-
-
-
?
Other publictions for EC 3.5.3.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
209433
Nakajima
A new amidinohydrolase, methyl ...
Alcaligenes sp., Alcaligenes sp. N-42
FEBS Lett.
110
43-46
1980
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