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Literature summary for 3.5.3.15 extracted from

  • Shimizu, A.; Handa, K.; Honda, T.; Abe, N.; Kojima, T.; Takahara, H.
    Three isozymes of peptidylarginine deiminase in the chicken: molecular cloning, characterization, and tissue distribution (2014), Comp. Biochem. Physiol. B, 167, 65-73.
    View publication on PubMed
Search for more literature for 3.5.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf21 insect cells Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73700
-
 x * 73700, isoform PAD1, SDS-PAGE Gallus gallus
74200
-
 x * 74200, isoform PAD2, SDS-PAGE Gallus gallus
74500
-
 x * 74500, isoform PAD3, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein L-arginine + H2O Gallus gallus
-
 protein L-citrulline + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus E1C0W6 isoform PAD1
-
Gallus gallus F1NP39 isoform PAD3
-
Gallus gallus S6BNL5 isoform PAD2
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
cerebellum
-
 Gallus gallus
-
cerebrum
-
 Gallus gallus
-
gizzard
-
 Gallus gallus
-
glandular stomach
-
 Gallus gallus
-
heart
-
 Gallus gallus
-
liver
-
 Gallus gallus
-
lung
-
 Gallus gallus
-
additional information isoform PAD2 protein is not detected in lung, heart, liver, granular stomach, and gizzard Gallus gallus
-
muscle
-
 Gallus gallus
-
optic lobe
-
 Gallus gallus
-
retina
-
 Gallus gallus
-
spinal cord
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine-methyl ester + H2O isoform PAD1 shows 15% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus L-citrulline methyl ester + NH3
-
 ?
L-arginine-methyl ester + H2O isoform PAD3 shows 15% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus L-citrulline methyl ester + NH3
-
 ?
additional information no activity with L-arginine Gallus gallus ?
-
 ?
N-acetyl-L-arginine + H2O isoform PAD1 shows 160% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus N-acetyl-L-citrulline ethyl ester + NH3
-
 ?
N-acetyl-L-arginine + H2O isoform PAD3 shows 135% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus N-acetyl-L-citrulline ethyl ester + NH3
-
 ?
N-acetyl-L-arginine-methyl ester + H2O isoform PAD1 shows 80% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus N-acetyl-L-citrulline-methyl ester + NH3
-
 ?
N-acetyl-L-arginine-methyl ester + H2O isoform PAD3 shows 125% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus N-acetyl-L-citrulline-methyl ester + NH3
-
 ?
Nalpha-benzoyl-L-arginine + H2O isoform PAD1 shows 120% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus Nalpha-benzoyl-L-citrulline + NH3
-
 ?
Nalpha-benzoyl-L-arginine + H2O isoform PAD3 shows 70% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus Nalpha-benzoyl-L-citrulline + NH3
-
 ?
Nalpha-benzoyl-L-arginine ethyl ester + H2O 100% activity Gallus gallus Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?
Nalpha-benzoyl-L-arginine-amide + H2O isoform PAD1 shows 110% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus Nalpha-benzoyl-L-citrulline-amide + NH3
-
 ?
Nalpha-benzoyl-L-arginine-amide + H2O isoform PAD3 shows 105% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus Nalpha-benzoyl-L-citrulline-amide + NH3
-
 ?
Nalpha-tosyl-L-arginine-methyl ester + H2O isoform PAD1 shows 20% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus Nalpha-tosyl-L-citrulline-methyl ester + NH3
-
 ?
Nalpha-tosyl-L-arginine-methyl ester + H2O isoform PAD3 shows 22% activity compared to Nalpha-benzoyl-L-arginine ethyl ester Gallus gallus Nalpha-tosyl-L-citrulline-methyl ester + NH3
-
 ?
protein L-arginine + H2O
-
 Gallus gallus protein L-citrulline + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 73700, isoform PAD1, SDS-PAGE Gallus gallus
? x * 74200, isoform PAD2, SDS-PAGE Gallus gallus
? x * 74500, isoform PAD3, SDS-PAGE Gallus gallus

Synonyms

Synonyms Comment Organism
PAD1 isoform Gallus gallus
PAD2 inactive isoform Gallus gallus
PAD3 isoform Gallus gallus
peptidylarginine deiminase
-
 Gallus gallus