Cloned (Comment) | Organism |
---|---|
expressed in Sf21 insect cells | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73700 | - |
x * 73700, isoform PAD1, SDS-PAGE | Gallus gallus |
74200 | - |
x * 74200, isoform PAD2, SDS-PAGE | Gallus gallus |
74500 | - |
x * 74500, isoform PAD3, SDS-PAGE | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein L-arginine + H2O | Gallus gallus | - |
protein L-citrulline + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | E1C0W6 | isoform PAD1 | - |
Gallus gallus | F1NP39 | isoform PAD3 | - |
Gallus gallus | S6BNL5 | isoform PAD2 | - |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cerebellum | - |
Gallus gallus | - |
cerebrum | - |
Gallus gallus | - |
gizzard | - |
Gallus gallus | - |
glandular stomach | - |
Gallus gallus | - |
heart | - |
Gallus gallus | - |
liver | - |
Gallus gallus | - |
lung | - |
Gallus gallus | - |
additional information | isoform PAD2 protein is not detected in lung, heart, liver, granular stomach, and gizzard | Gallus gallus | - |
muscle | - |
Gallus gallus | - |
optic lobe | - |
Gallus gallus | - |
retina | - |
Gallus gallus | - |
spinal cord | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine-methyl ester + H2O | isoform PAD1 shows 15% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | L-citrulline methyl ester + NH3 | - |
? | |
L-arginine-methyl ester + H2O | isoform PAD3 shows 15% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | L-citrulline methyl ester + NH3 | - |
? | |
additional information | no activity with L-arginine | Gallus gallus | ? | - |
? | |
N-acetyl-L-arginine + H2O | isoform PAD1 shows 160% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | N-acetyl-L-citrulline ethyl ester + NH3 | - |
? | |
N-acetyl-L-arginine + H2O | isoform PAD3 shows 135% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | N-acetyl-L-citrulline ethyl ester + NH3 | - |
? | |
N-acetyl-L-arginine-methyl ester + H2O | isoform PAD1 shows 80% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | N-acetyl-L-citrulline-methyl ester + NH3 | - |
? | |
N-acetyl-L-arginine-methyl ester + H2O | isoform PAD3 shows 125% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | N-acetyl-L-citrulline-methyl ester + NH3 | - |
? | |
Nalpha-benzoyl-L-arginine + H2O | isoform PAD1 shows 120% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | Nalpha-benzoyl-L-citrulline + NH3 | - |
? | |
Nalpha-benzoyl-L-arginine + H2O | isoform PAD3 shows 70% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | Nalpha-benzoyl-L-citrulline + NH3 | - |
? | |
Nalpha-benzoyl-L-arginine ethyl ester + H2O | 100% activity | Gallus gallus | Nalpha-benzoyl-L-citrulline ethyl ester + NH3 | - |
? | |
Nalpha-benzoyl-L-arginine-amide + H2O | isoform PAD1 shows 110% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | Nalpha-benzoyl-L-citrulline-amide + NH3 | - |
? | |
Nalpha-benzoyl-L-arginine-amide + H2O | isoform PAD3 shows 105% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | Nalpha-benzoyl-L-citrulline-amide + NH3 | - |
? | |
Nalpha-tosyl-L-arginine-methyl ester + H2O | isoform PAD1 shows 20% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | Nalpha-tosyl-L-citrulline-methyl ester + NH3 | - |
? | |
Nalpha-tosyl-L-arginine-methyl ester + H2O | isoform PAD3 shows 22% activity compared to Nalpha-benzoyl-L-arginine ethyl ester | Gallus gallus | Nalpha-tosyl-L-citrulline-methyl ester + NH3 | - |
? | |
protein L-arginine + H2O | - |
Gallus gallus | protein L-citrulline + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 73700, isoform PAD1, SDS-PAGE | Gallus gallus |
? | x * 74200, isoform PAD2, SDS-PAGE | Gallus gallus |
? | x * 74500, isoform PAD3, SDS-PAGE | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
PAD1 | isoform | Gallus gallus |
PAD2 | inactive isoform | Gallus gallus |
PAD3 | isoform | Gallus gallus |
peptidylarginine deiminase | - |
Gallus gallus |