Protein Variants | Comment | Organism |
---|---|---|
A112G | K0.5 for Ca2+ higher than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
A82V | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R123K | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) comparable to wild-type | Homo sapiens |
R123Q | K0.5 for Ca2+ higher than that obtained with the R123K mutant, but lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) comparable to wild-type | Homo sapiens |
R156K | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R156Q | K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R205K | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R205Q | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R372K | 830fold decreased in kcat/M with either substrate [histone H4]-L-Arg or Nalpha-benzoyl L-arginine ethyl ester | Homo sapiens |
R372Q | 830fold decreased in kcat/M with either substrate [histone H4]-L-Arg or Nalpha-benzoyl L-arginine ethyl ester | Homo sapiens |
R374K | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R374Q | K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R419K | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) comparable to wild-type | Homo sapiens |
R419Q | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R484K | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R484Q | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R609K | K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R609Q | K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lcomparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R639K | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
R639Q | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type | Homo sapiens |
S55G | K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) higher than wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R374K | Homo sapiens | |
0.094 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R639Q | Homo sapiens | |
0.1 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R123Q | Homo sapiens | |
0.11 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R156Q | Homo sapiens | |
0.14 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R419K | Homo sapiens | |
0.15 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R374Q | Homo sapiens | |
0.15 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R639K | Homo sapiens | |
0.17 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant A112G | Homo sapiens | |
0.18 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R156K | Homo sapiens | |
0.18 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R609Q | Homo sapiens | |
0.2 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant S55G | Homo sapiens | |
0.21 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, wild-type | Homo sapiens | |
0.22 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R419Q | Homo sapiens | |
0.24 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R123K | Homo sapiens | |
0.26 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R484K | Homo sapiens | |
0.3 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R484Q | Homo sapiens | |
0.35 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant A82V | Homo sapiens | |
0.44 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant S55G | Homo sapiens | |
0.45 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R484K | Homo sapiens | |
0.45 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R484Q | Homo sapiens | |
0.48 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R205Q | Homo sapiens | |
0.5 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R156K | Homo sapiens | |
0.58 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, wild-type | Homo sapiens | |
0.69 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R639K | Homo sapiens | |
0.72 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R419K | Homo sapiens | |
0.8 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant A112G | Homo sapiens | |
0.86 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R123K | Homo sapiens | |
0.87 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R419Q | Homo sapiens | |
0.89 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R609Q | Homo sapiens | |
1.2 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R374Q | Homo sapiens | |
1.2 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R639Q | Homo sapiens | |
1.35 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant A82V | Homo sapiens | |
1.4 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R123Q | Homo sapiens | |
1.5 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R205K | Homo sapiens | |
1.5 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R609K | Homo sapiens | |
1.6 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R156Q | Homo sapiens | |
1.7 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R374K | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the binding affinity of autodeiminated PAD4 for p53 and H3 are similar to non-deiminated PAD4. In contrast, the affinity of autodeiminated PAD4 for HDAC1, PRMT1, and Cit H3 is reduced by 3.7, 4.5, and 10fold, respectively. Results suggest that the autodeimination of PAD4 in vivo could affect its functional activity by regulating its ability to interact with binding partners | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | PAD4 autodeimination does not alter the activity, substrate specificity, or calcium dependence of this isozyme. Six in vitro sites of autodeimination are identified: R123, R156, R205, R419, R484, and R639. Autodeimination of PAD4 reduces protein-protein interactions | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the binding affinity of autodeiminated PAD4 for p53 and H3 are similar to non-deiminated PAD4. In contrast, the affinity of autodeiminated PAD4 for HDAC1, PRMT1, and Cit H3 is reduced by 3.7, 4.5, and 10fold, respectively. Results suggest that the autodeimination of PAD4 in vivo could affect its functional activity by regulating its ability to interact with binding partners | Homo sapiens | ? | - |
? | |
Nalpha-benzoyl L-arginine ethyl ester + H2O | - |
Homo sapiens | Nalpha-benzoyl L-citrulline ethyl ester + NH3 | - |
? | |
PAD4-L-arginine + H2O | PAD4 is autodeiminated in a time-dependent manner | Homo sapiens | PAD4-L-citrulline + NH3 | - |
? | |
[histone H4]-L-Arg + H2O | - |
Homo sapiens | [histone H4]-L-citrulline + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAD4 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R374K | Homo sapiens | |
0.55 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R419Q | Homo sapiens | |
0.76 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R156K | Homo sapiens | |
0.77 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R374Q | Homo sapiens | |
0.78 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R639Q | Homo sapiens | |
0.79 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R156Q | Homo sapiens | |
0.9 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R123Q | Homo sapiens | |
1 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant A112G | Homo sapiens | |
1.1 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R484K | Homo sapiens | |
1.1 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant S55G | Homo sapiens | |
1.2 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R205Q | Homo sapiens | |
1.2 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R419Q | Homo sapiens | |
1.2 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R484Q | Homo sapiens | |
1.3 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R419K | Homo sapiens | |
1.3 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R639K | Homo sapiens | |
1.4 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant A82V | Homo sapiens | |
1.4 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R156K | Homo sapiens | |
1.5 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant A112G | Homo sapiens | |
1.5 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R609Q | Homo sapiens | |
1.6 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R484K | Homo sapiens | |
1.6 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R484Q | Homo sapiens | |
1.6 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R639Q | Homo sapiens | |
1.7 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R123K | Homo sapiens | |
1.9 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R609Q | Homo sapiens | |
1.9 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R639K | Homo sapiens | |
2.1 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, wild-type | Homo sapiens | |
2.3 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R123Q | Homo sapiens | |
2.3 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R374Q | Homo sapiens | |
2.6 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R609K | Homo sapiens | |
2.7 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant A82V | Homo sapiens | |
2.8 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant S55G | Homo sapiens | |
2.8 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, wild-type | Homo sapiens | |
3 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R374K | Homo sapiens | |
3.1 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R419K | Homo sapiens | |
3.6 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R156Q | Homo sapiens | |
3.7 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R123K | Homo sapiens | |
3.8 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R205K | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R372K, value below | Homo sapiens | |
0.012 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R372Q, value below | Homo sapiens | |
0.63 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R419Q | Homo sapiens | |
1.3 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R639Q | Homo sapiens | |
1.5 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R156K | Homo sapiens | |
1.6 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R123Q | Homo sapiens | |
1.7 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R609K | Homo sapiens | |
1.8 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R374K | Homo sapiens | |
1.9 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant A112G | Homo sapiens | |
1.9 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R374Q | Homo sapiens | |
2.1 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant A82V | Homo sapiens | |
2.1 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R205Q | Homo sapiens | |
2.1 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R609Q | Homo sapiens | |
2.2 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R156Q | Homo sapiens | |
2.4 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R484K | Homo sapiens | |
2.5 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R205K | Homo sapiens | |
2.5 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R205Q | Homo sapiens | |
2.7 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R484Q | Homo sapiens | |
2.8 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R639K | Homo sapiens | |
3.7 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R205K | Homo sapiens | |
4 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant A82V | Homo sapiens | |
4.3 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R123K | Homo sapiens | |
4.3 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant R419K | Homo sapiens | |
4.8 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, wild-type | Homo sapiens | |
4.9 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R609K | Homo sapiens | |
5.1 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R374Q | Homo sapiens | |
5.3 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R484Q | Homo sapiens | |
5.5 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R419Q | Homo sapiens | |
5.5 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant S55G | Homo sapiens | |
5.6 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R374K | Homo sapiens | |
5.9 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant A112G | Homo sapiens | |
6.2 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R484K | Homo sapiens | |
6.4 | - |
Nalpha-benzoyl L-arginine ethyl ester | 37°C, pH 7.6, mutant S55G | Homo sapiens | |
7.1 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R123K | Homo sapiens | |
7.2 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R156Q | Homo sapiens | |
7.8 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R156K | Homo sapiens | |
8.3 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R609Q | Homo sapiens | |
8.3 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R639Q | Homo sapiens | |
8.7 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R639K | Homo sapiens | |
9 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R123Q | Homo sapiens | |
9.3 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, mutant R419K | Homo sapiens | |
10 | - |
[histone H4]-L-Arg | 37°C, pH 7.6, wild-type | Homo sapiens |