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Literature summary for 3.5.3.15 extracted from

  • Slack, J.L.; Jones, L.E.; Bhatia, M.M.; Thompson, P.R.
    Autodeimination of protein arginine deiminase 4 alters protein-protein interactions but not activity (2011), Biochemistry, 50, 3997-4010.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A112G K0.5 for Ca2+ higher than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
A82V K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R123K K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) comparable to wild-type Homo sapiens
R123Q K0.5 for Ca2+ higher than that obtained with the R123K mutant, but lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) comparable to wild-type Homo sapiens
R156K K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R156Q K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R205K K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R205Q K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R372K 830fold decreased in kcat/M with either substrate [histone H4]-L-Arg or Nalpha-benzoyl L-arginine ethyl ester Homo sapiens
R372Q 830fold decreased in kcat/M with either substrate [histone H4]-L-Arg or Nalpha-benzoyl L-arginine ethyl ester Homo sapiens
R374K K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R374Q K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R419K K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) comparable to wild-type Homo sapiens
R419Q K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R484K K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R484Q K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R609K K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R609Q K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lcomparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R639K K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
R639Q K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type Homo sapiens
S55G K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) higher than wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.088
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R374K Homo sapiens
0.094
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R639Q Homo sapiens
0.1
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R123Q Homo sapiens
0.11
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R156Q Homo sapiens
0.14
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R419K Homo sapiens
0.15
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R374Q Homo sapiens
0.15
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R639K Homo sapiens
0.17
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant A112G Homo sapiens
0.18
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R156K Homo sapiens
0.18
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R609Q Homo sapiens
0.2
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant S55G Homo sapiens
0.21
-
[histone H4]-L-Arg 37°C, pH 7.6, wild-type Homo sapiens
0.22
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R419Q Homo sapiens
0.24
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R123K Homo sapiens
0.26
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R484K Homo sapiens
0.3
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R484Q Homo sapiens
0.35
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant A82V Homo sapiens
0.44
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant S55G Homo sapiens
0.45
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R484K Homo sapiens
0.45
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R484Q Homo sapiens
0.48
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R205Q Homo sapiens
0.5
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R156K Homo sapiens
0.58
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, wild-type Homo sapiens
0.69
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R639K Homo sapiens
0.72
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R419K Homo sapiens
0.8
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant A112G Homo sapiens
0.86
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R123K Homo sapiens
0.87
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R419Q Homo sapiens
0.89
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R609Q Homo sapiens
1.2
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R374Q Homo sapiens
1.2
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R639Q Homo sapiens
1.35
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant A82V Homo sapiens
1.4
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R123Q Homo sapiens
1.5
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R205K Homo sapiens
1.5
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R609K Homo sapiens
1.6
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R156Q Homo sapiens
1.7
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R374K Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the binding affinity of autodeiminated PAD4 for p53 and H3 are similar to non-deiminated PAD4. In contrast, the affinity of autodeiminated PAD4 for HDAC1, PRMT1, and Cit H3 is reduced by 3.7, 4.5, and 10fold, respectively. Results suggest that the autodeimination of PAD4 in vivo could affect its functional activity by regulating its ability to interact with binding partners ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification PAD4 autodeimination does not alter the activity, substrate specificity, or calcium dependence of this isozyme. Six in vitro sites of autodeimination are identified: R123, R156, R205, R419, R484, and R639. Autodeimination of PAD4 reduces protein-protein interactions Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the binding affinity of autodeiminated PAD4 for p53 and H3 are similar to non-deiminated PAD4. In contrast, the affinity of autodeiminated PAD4 for HDAC1, PRMT1, and Cit H3 is reduced by 3.7, 4.5, and 10fold, respectively. Results suggest that the autodeimination of PAD4 in vivo could affect its functional activity by regulating its ability to interact with binding partners Homo sapiens ?
-
?
Nalpha-benzoyl L-arginine ethyl ester + H2O
-
Homo sapiens Nalpha-benzoyl L-citrulline ethyl ester + NH3
-
?
PAD4-L-arginine + H2O PAD4 is autodeiminated in a time-dependent manner Homo sapiens PAD4-L-citrulline + NH3
-
?
[histone H4]-L-Arg + H2O
-
Homo sapiens [histone H4]-L-citrulline + NH3
-
?

Synonyms

Synonyms Comment Organism
PAD4
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.49
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R374K Homo sapiens
0.55
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R419Q Homo sapiens
0.76
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R156K Homo sapiens
0.77
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R374Q Homo sapiens
0.78
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R639Q Homo sapiens
0.79
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R156Q Homo sapiens
0.9
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R123Q Homo sapiens
1
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant A112G Homo sapiens
1.1
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R484K Homo sapiens
1.1
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant S55G Homo sapiens
1.2
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R205Q Homo sapiens
1.2
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R419Q Homo sapiens
1.2
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R484Q Homo sapiens
1.3
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R419K Homo sapiens
1.3
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R639K Homo sapiens
1.4
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant A82V Homo sapiens
1.4
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R156K Homo sapiens
1.5
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant A112G Homo sapiens
1.5
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R609Q Homo sapiens
1.6
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R484K Homo sapiens
1.6
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R484Q Homo sapiens
1.6
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R639Q Homo sapiens
1.7
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R123K Homo sapiens
1.9
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R609Q Homo sapiens
1.9
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R639K Homo sapiens
2.1
-
[histone H4]-L-Arg 37°C, pH 7.6, wild-type Homo sapiens
2.3
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R123Q Homo sapiens
2.3
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R374Q Homo sapiens
2.6
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R609K Homo sapiens
2.7
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant A82V Homo sapiens
2.8
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant S55G Homo sapiens
2.8
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, wild-type Homo sapiens
3
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R374K Homo sapiens
3.1
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R419K Homo sapiens
3.6
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R156Q Homo sapiens
3.7
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R123K Homo sapiens
3.8
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R205K Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0031
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R372K, value below Homo sapiens
0.012
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R372Q, value below Homo sapiens
0.63
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R419Q Homo sapiens
1.3
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R639Q Homo sapiens
1.5
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R156K Homo sapiens
1.6
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R123Q Homo sapiens
1.7
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R609K Homo sapiens
1.8
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R374K Homo sapiens
1.9
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant A112G Homo sapiens
1.9
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R374Q Homo sapiens
2.1
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant A82V Homo sapiens
2.1
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R205Q Homo sapiens
2.1
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R609Q Homo sapiens
2.2
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R156Q Homo sapiens
2.4
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R484K Homo sapiens
2.5
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R205K Homo sapiens
2.5
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R205Q Homo sapiens
2.7
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R484Q Homo sapiens
2.8
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R639K Homo sapiens
3.7
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R205K Homo sapiens
4
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant A82V Homo sapiens
4.3
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R123K Homo sapiens
4.3
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant R419K Homo sapiens
4.8
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, wild-type Homo sapiens
4.9
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R609K Homo sapiens
5.1
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R374Q Homo sapiens
5.3
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R484Q Homo sapiens
5.5
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R419Q Homo sapiens
5.5
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant S55G Homo sapiens
5.6
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R374K Homo sapiens
5.9
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant A112G Homo sapiens
6.2
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R484K Homo sapiens
6.4
-
Nalpha-benzoyl L-arginine ethyl ester 37°C, pH 7.6, mutant S55G Homo sapiens
7.1
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R123K Homo sapiens
7.2
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R156Q Homo sapiens
7.8
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R156K Homo sapiens
8.3
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R609Q Homo sapiens
8.3
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R639Q Homo sapiens
8.7
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R639K Homo sapiens
9
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R123Q Homo sapiens
9.3
-
[histone H4]-L-Arg 37°C, pH 7.6, mutant R419K Homo sapiens
10
-
[histone H4]-L-Arg 37°C, pH 7.6, wild-type Homo sapiens