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Literature summary for 3.5.3.15 extracted from

  • Stensland, M.E.; Pollmann, S.; Molberg, ?.; Sollid, L.M.; Fleckenstein, B.
    Primary sequence, together with other factors, influence peptide deimination by peptidylarginine deiminase-4 (2009), Biol. Chem., 390, 99-107.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells as a fusion protein with glutathione S-transferase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
WSRYH in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
0.187
-
YWRDH in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
0.27
-
MWRHV in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
0.325
-
KDRNW in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
0.345
-
WTRGE in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
0.372
-
TKQTARKSTGGK in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
0.45
-
LDRGE in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
0.575
-
TSTGGRQGSHH in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens a methylated lysine residue flanking the targeted arginine influences PAD-4-mediated deimination ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose 4B column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
filaggrin L-Arg + H2O
-
Homo sapiens filaggrin L-citrulline + NH3
-
?
histone H3 L-arginine + H2O
-
Homo sapiens histone H3 L-citrulline + NH3
-
?
KDRNW + H2O
-
Homo sapiens ?
-
?
LDRGE + H2O
-
Homo sapiens ?
-
?
additional information a methylated lysine residue flanking the targeted arginine influences PAD-4-mediated deimination Homo sapiens ?
-
?
additional information no activity toward PVRAK, DQRTA, DVRIA, and EPRLF Homo sapiens ?
-
?
MWRHV + H2O
-
Homo sapiens ?
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
Homo sapiens N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
?
TKQTARKSTGGK + H2O
-
Homo sapiens ?
-
?
TSTGGRQGSHH + H2O
-
Homo sapiens ?
-
?
WSRYH + H2O
-
Homo sapiens ?
-
?
WTRGE + H2O
-
Homo sapiens ?
-
?
YWRDH + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
PAD-4
-
Homo sapiens
peptidylarginine deiminase-4
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
TKQTARKSTGGK in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
1.3
-
TSTGGRQGSHH in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
1.8
-
WSRYH in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
2
-
MWRHV in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
2
-
KDRNW in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
2.3
-
YWRDH in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
2.9
-
LDRGE in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens
3.3
-
WTRGE in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C Homo sapiens