Literature summary for 3.5.3.15 extracted from

Wood, D.D.; Ackerley, C.A.; Brand, B.; Zhang, L.; Raijmakers, R.; Mastronardi, F.G.; Moscarello, M.A.
Myelin localization of peptidylarginine deiminases 2 and 4: comparison of PAD2 and PAD4 activities (2008), Lab. Invest., 88, 354-364.

Search for more literature for 3.5.3.15

Activating Compound

Activating Compound	Comment	Organism	Structure
lipid vesicle	binding of substrate myelin basic protein to lipid vesicles, consisting of 7.8% phosphatidylserine and 92.2% phosphatidylcholine, increases deimination activity, but in the 40 amino acid residue C-terminal region, where the arginyl residues are no longer deiminated, while they are deiminated in aqueous solution	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
additional information	inactivation of PAD2 does not affect PAD4 activity, but leads to limited deimination due to the narrower cleavage spectrum of arginyl residues in myelin basic protein for PAD4, overview	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
lipid vesicle	binding of substrate myelin basic protein to lipid vesicles, consisting of 7.8% phosphatidylserine and 92.2% phosphatidylcholine, increases deimination activity, but in the 40 mino acid C-terminal region, where the arginyl residues are no longer deiminated, while they are deiminated in aqueous solution; binding of substrate myelin basic protein to lipid vesicles, consisting of 7.8% phosphatidylserine and 92.2% phosphatidylcholine, increases deimination activity, but in the 40 mino acid C-terminal region, where the arginyl residues are no longer deiminated, while they are deiminated in aqueous solution	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Mus musculus	5829	-
cytosol	PAD2	Mus musculus	5829	-
nucleus	PAD4 associated with chromatin and with intermediate filaments in the axon	Mus musculus	5634	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
myelin basic protein-L-arginine + H2O	Mus musculus	-	myelin basic protein-L-citrulline + NH3	-	?
myelin basic protein-L-arginine + H2O	Homo sapiens	PAD2 and PAD4 are involved in the pathogenesis of demyelination diseases such as multiple sclerosis, overview	myelin basic protein-L-citrulline + NH3	-	?
myelin basic protein-L-arginine + H2O	Mus musculus CD-1	-	myelin basic protein-L-citrulline + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Mus musculus	Q08642	PAD2; CD-1 mice	-
Mus musculus	Q9Z183	PAD4; CD-1 mice	-
Mus musculus CD-1	Q08642	PAD2; CD-1 mice	-
Mus musculus CD-1	Q9Z183	PAD4; CD-1 mice	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Mus musculus	-
brain	of healthy persons and of multiple sclerosis patients	Homo sapiens	-
cerebral white matter	-	Homo sapiens	-
cerebral white matter	-	Mus musculus	-
myelin sheath	-	Mus musculus	-
myelin sheath	quantitative determination of PAD2 and PAD4 by immunohistochemistry	Homo sapiens	-
oligodendrocyte	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myelin basic protein-L-arginine + H2O	-	Mus musculus	myelin basic protein-L-citrulline + NH3	-	?
myelin basic protein-L-arginine + H2O	PAD2 and PAD4 are involved in the pathogenesis of demyelination diseases such as multiple sclerosis, overview	Homo sapiens	myelin basic protein-L-citrulline + NH3	-	?
myelin basic protein-L-arginine + H2O	amino acid sequence determination of the substrate protein by LC/MS, PAD2 deiminates 18 of 19 arginyl residues, whereas PAD4 deiminates 14 of 19 arginyl residues, determination of deimination residues, overview	Homo sapiens	myelin basic protein-L-citrulline + NH3	-	?
myelin basic protein-L-arginine + H2O	-	Mus musculus CD-1	myelin basic protein-L-citrulline + NH3	-	?

Synonyms

Synonyms	Comment	Organism
PAD2	-	Homo sapiens
PAD2	-	Mus musculus
PAD4	-	Homo sapiens
PAD4	-	Mus musculus
peptidylarginine deiminase	-	Homo sapiens
peptidylarginine deiminase	-	Mus musculus
peptidylarginine deiminase 4	-	Homo sapiens
peptidylarginine deiminase 4	-	Mus musculus

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Release 2023.1 (January 2023)