Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.3.15 extracted from

  • Ohsugi, I.; Takahara, H.; Shiraiwa, M.; Sugawara, K.
    Expression of mouse uterine peptidylarginine deiminase in Escherichia coli: construction of expression plasmid and properties of the recombinant enzyme (1995), Arch. Biochem. Biophys., 317, 62-68.
    View publication on PubMed
Search for more literature for 3.5.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 benzoyl-Arg ethyl ester native enzyme Mus musculus
0.53
-
 benzoyl-L-Arg methyl ester recombinant enzyme Mus musculus
0.54
-
 benzoyl-L-Arg methyl ester native enzyme Mus musculus
0.54
-
 benzoyl-Arg ethyl ester recombinant enzyme Mus musculus
1.37
-
 benzoyl-L-Arg-NH2 native enzyme Mus musculus
1.39
-
 benzoyl-L-Arg-NH2 recombinant enzyme Mus musculus
2.28
-
 benzoyl-L-Arg native enzyme Mus musculus
2.58
-
 benzoyl-L-Arg recombinant enzyme Mus musculus
2.96
-
 acetyl-L-Arg methyl ester native enzyme Mus musculus
3.24
-
 acetyl-L-Arg methyl ester recombinant enzyme Mus musculus
10.67
-
 acetyl-L-Arg recombinant enzyme Mus musculus
11.3
-
 acetyl-L-Arg native enzyme Mus musculus
23.49
-
 L-Arg methyl ester recombinant enzyme Mus musculus
26.45
-
 L-Arg methyl ester native enzyme Mus musculus
33.4
-
 L-Arg native enzyme Mus musculus
36.3
-
 L-Arg recombinant enzyme Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximal activity at 2 mM Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle skeletal muscle Mus musculus
-
uterus
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.45
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acety-L-Arg + H2O
-
 Mus musculus acetyl-L-citrulline + NH3
-
 ?
acetyl-L-Arg + H2O
-
 Mus musculus acetyl-L-citrulline + NH3
-
 ?
benzoyl-Arg ethyl ester + H2O
-
 Mus musculus benzoyl-citrulline ethyl ester + NH3
-
 ?
benzoyl-Arg methyl ester + H2O
-
 Mus musculus benzoyl-citrulline methyl ester + NH3
-
 ?
benzoyl-L-Arg + H2O
-
 Mus musculus benzoyl-L-citrulline + NH3
-
 ?
L-Arg + H2O
-
 Mus musculus L-citrulline + NH3
-
 ?
L-Arg methyl ester + H2O
-
 Mus musculus L-citrulline methyl ester + NH4+
-
 ?
N-acetyl-Arg methyl ester + H2O
-
 Mus musculus N-acetyl-citrulline + NH4+
-
 ?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
-
 Mus musculus Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
 L-Arg methyl ester recombinant enzyme Mus musculus
0.22
-
 L-Arg recombinant enzyme Mus musculus
0.31
-
 L-Arg methyl ester native enzyme Mus musculus
0.32
-
 acetyl-L-Arg recombinant enzyme Mus musculus
0.37
-
 acetyl-L-Arg native enzyme Mus musculus
0.52
-
 L-Arg native enzyme Mus musculus
1.11
-
 benzoyl-L-Arg native enzyme Mus musculus
1.41
-
 benzoyl-L-Arg recombinant enzyme Mus musculus
4.11
-
 acetyl-L-Arg methyl ester native enzyme Mus musculus
4.41
-
 acetyl-L-Arg methyl ester recombinant enzyme Mus musculus
5.31
-
 benzoyl-L-Arg-NH2 recombinant enzyme Mus musculus
5.51
-
 benzoyl-L-Arg-NH2 native enzyme Mus musculus
5.69
-
 benzoyl-L-Arg methyl ester recombinant enzyme Mus musculus
5.74
-
 benzoyl-L-Arg methyl ester native enzyme Mus musculus
10.7
-
 benzoyl-Arg ethyl ester recombinant enzyme Mus musculus
10.8
-
 benzoyl-arginine ethyl ester native enzyme Mus musculus