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Literature summary for 3.5.3.14 extracted from

  • Milstien, S.; Goldman, P.
    Metabolism of guanidinosuccinic acid. I. Characterization of a specific amidinohydrolase from Pseudomonas chlororaphis (1972), J. Biol. Chem., 247, 6280-6283.
    View publication on PubMed
Search for more literature for 3.5.3.14

Application

Application Comment Organism
medicine test for N-amidino-L-aspartate in the urine of uremic patients Pseudomonas chlororaphis

Inhibitors

Inhibitors Comment Organism Structure
aspartic acid competitive inhibition Pseudomonas chlororaphis
Urea competitive inhibition Pseudomonas chlororaphis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 N-amidino-L-aspartate HEPES buffer, pH 8, assay method B Pseudomonas chlororaphis
8.3
-
 N-amidino-L-aspartate HEPES buffer, pH 8, assay method A Pseudomonas chlororaphis
20
-
 N-amidino-L-aspartate phosphate buffer Pseudomonas chlororaphis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Pseudomonas putida
Co2+ temperature-dependent activation Pseudomonas chlororaphis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
 gel filtration Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-amidino-L-aspartic acid Pseudomonas putida
-
 L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid Pseudomonas chlororaphis
-
 L-aspartic acid + urea
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis
-
 fluorescent Pseudomonas
-
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.73
-
-
 Pseudomonas chlororaphis

Storage Stability

Storage Stability Organism
-15°C, stable for several months Pseudomonas chlororaphis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanidinoglutaric acid + H2O
-
 Pseudomonas chlororaphis ?
-
 ?
additional information
-
 Pseudomonas putida ?
-
 ?
additional information enzyme is specific for the L-isomer Pseudomonas chlororaphis ?
-
 ?
additional information N-amidino-D-aspartate, 3-guanidinopropionic acid, 4-guanidinobutyric acid, 2-amino-4-guanidinopropionic acid, agmatine, arginine, N-acetyl-arginine, creatinine, guanidinoacetic acid are no substrates Pseudomonas chlororaphis ?
-
 ?
N-amidino-L-aspartic acid
-
 Pseudomonas putida L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid
-
 Pseudomonas chlororaphis L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid + H2O
-
 Pseudomonas putida L-aspartic acid + urea
-
 ?
N-amidino-L-aspartic acid + H2O
-
 Pseudomonas chlororaphis L-aspartic acid + urea
-
 ?
N-amidino-L-glutamate + H2O
-
 Pseudomonas putida L-glutamic acid + urea
-
 ?
N-amidino-L-glutamate + H2O
-
 Pseudomonas chlororaphis L-glutamic acid + urea
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 incubation at 40°C results in rapid activation, prolonged incubation causes irreversible loss of activity, incubation at 50°C 90% of the initial enzyme activity lost within 10 min Pseudomonas chlororaphis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas chlororaphis