Reference to 3.5.3.13; Id = 664125 - BRENDA Enzyme Database

expression in Escherichia coli

Pseudomonas aeruginosa

additional information

no inhibition by N-acetyl-L-glutamate, N-carbamoyl-L-glutamate, N-formyl-L-glutamate, N-formimino-alpha-aminobutyric acid and N-formimino-gamma-aminobutyric acid at concentrations up to 10 mM

Pseudomonas aeruginosa

N-formimino-L-aspartate

-

Pseudomonas aeruginosa

N-guanidino-L-glutamate

-

Pseudomonas aeruginosa

0.0012

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Cu2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.0021

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Cd2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.0027

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Ni2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.22

-

N-formimino-L-glutamate

wild-type, pH 8.0, 30Â°C

Pseudomonas aeruginosa

Zn2+

bound to the protein

Pseudomonas aeruginosa

49000

-

2 * 49000, SDS-PAGE, gel filtration

Pseudomonas aeruginosa

100000

-

gel filtration

Pseudomonas aeruginosa

-

Pseudomonas aeruginosa

N-formimino-alpha-aminobutyric acid + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-alpha-aminobutyric acid + NH3

-

?

N-formimino-D-glutamate + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-D-glutamate + NH3

-

?

N-formimino-gamma-aminobutyric acid + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-gamma-aminobutyric acid + NH3

-

?

N-formimino-glycine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-glycine + NH3

-

?

N-formimino-L-alanine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-alanine + NH3

-

?

N-formimino-L-aspartate + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-aspartate + NH3

-

?

N-formimino-L-glutamate + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-glutamate + NH3

-

?

N-formimino-L-isoleucine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-isoleucine + NH3

-

?

N-formimino-L-leucine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-leucine + NH3

-

?

N-formimino-L-methionine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-methionine + NH3

-

?

N-formimino-L-phenylalanine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-phenylalanine + NH3

-

?

N-formimino-L-tyrosine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-tyrosine + NH3

-

?

N-formimino-L-valine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-valine + NH3

-

?

N-guanidino-L-glutamate + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

?

-

?

dimer

2 * 49000, SDS-PAGE, gel filtration

Pseudomonas aeruginosa

N-formimino-L-glutamate deiminase

-

Pseudomonas aeruginosa

Pa5106

-

Pseudomonas aeruginosa

additional information

-

additional information

less than 0.001/sec for the substrates N-guanidino-L-glutamate, N-formimino-alpha-aminobutyric acid, N-formimino-gamma-aminobutyric acid, N-formimino-L-alanine, N-formimino-L-aspartate, N-formimino-glycine, N-formimino-L-leucine, N-formimino-L-phenylalanine, N-formimino-L-tyrosine, N-formimino-L-valine

Pseudomonas aeruginosa

0.01

-

N-formimino-L-methionine

pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.019

-

N-formimino-L-isoleucine

pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.05

-

N-formimino-D-glutamate

pH 8.0, 30Â°C

Pseudomonas aeruginosa

8.5

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Cu2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

13.2

-

N-formimino-L-glutamate

pH 8.0, 30Â°C

Pseudomonas aeruginosa

21.3

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Ni2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

31

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Cd2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.03

-

N-formimino-L-aspartate

pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.89

-

N-guanidino-L-glutamate

pH 8.0, 30Â°C

Pseudomonas aeruginosa

expression in Escherichia coli

Pseudomonas aeruginosa

additional information

no inhibition by N-acetyl-L-glutamate, N-carbamoyl-L-glutamate, N-formyl-L-glutamate, N-formimino-alpha-aminobutyric acid and N-formimino-gamma-aminobutyric acid at concentrations up to 10 mM

Pseudomonas aeruginosa

N-formimino-L-aspartate

-

Pseudomonas aeruginosa

N-guanidino-L-glutamate

-

Pseudomonas aeruginosa

0.03

-

N-formimino-L-aspartate

pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.89

-

N-guanidino-L-glutamate

pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.0012

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Cu2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.0021

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Cd2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.0027

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Ni2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.22

-

N-formimino-L-glutamate

wild-type, pH 8.0, 30Â°C

Pseudomonas aeruginosa

Zn2+

bound to the protein

Pseudomonas aeruginosa

49000

-

2 * 49000, SDS-PAGE, gel filtration

Pseudomonas aeruginosa

100000

-

gel filtration

Pseudomonas aeruginosa

-

Pseudomonas aeruginosa

N-formimino-alpha-aminobutyric acid + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-alpha-aminobutyric acid + NH3

-

?

N-formimino-D-glutamate + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-D-glutamate + NH3

-

?

N-formimino-gamma-aminobutyric acid + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-gamma-aminobutyric acid + NH3

-

?

N-formimino-glycine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-glycine + NH3

-

?

N-formimino-L-alanine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-alanine + NH3

-

?

N-formimino-L-aspartate + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-aspartate + NH3

-

?

N-formimino-L-glutamate + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-glutamate + NH3

-

?

N-formimino-L-isoleucine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-isoleucine + NH3

-

?

N-formimino-L-leucine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-leucine + NH3

-

?

N-formimino-L-methionine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-methionine + NH3

-

?

N-formimino-L-phenylalanine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-phenylalanine + NH3

-

?

N-formimino-L-tyrosine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-tyrosine + NH3

-

?

N-formimino-L-valine + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

N-formyl-L-valine + NH3

-

?

N-guanidino-L-glutamate + H2O

pH 8.0, 30Â°C

664125

Pseudomonas aeruginosa

?

-

?

dimer

2 * 49000, SDS-PAGE, gel filtration

Pseudomonas aeruginosa

additional information

-

additional information

less than 0.001/sec for the substrates N-guanidino-L-glutamate, N-formimino-alpha-aminobutyric acid, N-formimino-gamma-aminobutyric acid, N-formimino-L-alanine, N-formimino-L-aspartate, N-formimino-glycine, N-formimino-L-leucine, N-formimino-L-phenylalanine, N-formimino-L-tyrosine, N-formimino-L-valine

Pseudomonas aeruginosa

0.01

-

N-formimino-L-methionine

pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.019

-

N-formimino-L-isoleucine

pH 8.0, 30Â°C

Pseudomonas aeruginosa

0.05

-

N-formimino-D-glutamate

pH 8.0, 30Â°C

Pseudomonas aeruginosa

8.5

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Cu2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

13.2

-

N-formimino-L-glutamate

pH 8.0, 30Â°C

Pseudomonas aeruginosa

21.3

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Ni2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

31

-

N-formimino-L-glutamate

Zn2+ has been reconstituted with Cd2+, pH 8.0, 30Â°C

Pseudomonas aeruginosa

733363

Fedorov

Structure of N-formimino-L-glu ...

Pseudomonas aeruginosa

Biochemistry

54

890-897

2015

-

1

-

2

-

1

-

1

-

4

-

1

-

3

-

1

-

2

-

1

-

1

-

1

-

664125

Marti-Arbona

Annotating enzymes of unknown ...

Pseudomonas aeruginosa

Biochemistry

45

1997-2005

2006

-

1

-

3

4

-

1

2

-

4

-

1

-

14

1

2

-

8

-

2

-

1

-

3

2

4

-

1

2

-

1

-

14

1

-

8

-

685068

Marti-Arbona

Mechanistic characterization o ...

Pseudomonas aeruginosa

Biochemistry

45

14256-14262

2006

-

5

-

1

7

-

4

-

6

-

1

-

1

-

2

-

7

-

5

-

1

-

7

-

4

-

1

-

1

-

7

-

209406

Hu

Purification and properties of ...

Comamonas testosteroni, Pseudomonas aeruginosa, Pseudomonas putida, Streptomyces coelicolor

J. Bacteriol.

169

4696-4702

1987

-

1

-

4

-

4

-

9

-

8

-

2

-

1

-

4

-

9

-

8

-

172087

Kendrick

Histidine dissimilation in Str ...

Streptomyces coelicolor

J. Gen. Microbiol.

128

2029-2040

1982

-

1

-

3

-

2

-

1

-

1

-

2

-

209408

Wickner

N-Formimino-L-glutamate iminoh ...

Pseudomonas aeruginosa

J. Biol. Chem.

247

1605-1609

1972

-

1

-

6

1

-

3

-

1

-

1

-

3

-

1

-

1

-

6

1

-

1

-

1

-

3

-

209409

Wickner

-

N-Formimino-L-glutamate iminoh ...

Pseudomonas aeruginosa

Methods Enzymol.

17

80-84

1971

-

2

1

-

1

-

1

-

1

2

1

-

2

1

-

1

-

1

2

1

-

Annotating enzymes of unknown function: N-formimino-L-glutamate deiminase is a member of the amidohydrolase superfamily

Data extracted from this reference: