BRENDA - Enzyme Database
show all sequences of 3.5.3.13

Annotating enzymes of unknown function: N-formimino-L-glutamate deiminase is a member of the amidohydrolase superfamily

Marti-Arbona, R.; Xu, C.; Steele, S.; Weeks, A.; Kuty, G.F.; Seibert, C.M.; Raushel, F.M.; Biochemistry 45, 1997-2005 (2006) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Pseudomonas aeruginosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by N-acetyl-L-glutamate, N-carbamoyl-L-glutamate, N-formyl-L-glutamate, N-formimino-alpha-aminobutyric acid and N-formimino-gamma-aminobutyric acid at concentrations up to 10 mM
Pseudomonas aeruginosa
N-formimino-L-aspartate
-
Pseudomonas aeruginosa
N-guanidino-L-glutamate
-
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Cu2+, pH 8.0, 30°C
Pseudomonas aeruginosa
0.0021
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Cd2+, pH 8.0, 30°C
Pseudomonas aeruginosa
0.0027
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Ni2+, pH 8.0, 30°C
Pseudomonas aeruginosa
0.22
-
N-formimino-L-glutamate
wild-type, pH 8.0, 30°C
Pseudomonas aeruginosa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
bound to the protein
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
2 * 49000, SDS-PAGE, gel filtration
Pseudomonas aeruginosa
100000
-
gel filtration
Pseudomonas aeruginosa
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N-formimino-alpha-aminobutyric acid + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-alpha-aminobutyric acid + NH3
-
-
-
?
N-formimino-D-glutamate + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-D-glutamate + NH3
-
-
-
?
N-formimino-gamma-aminobutyric acid + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-gamma-aminobutyric acid + NH3
-
-
-
?
N-formimino-glycine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-glycine + NH3
-
-
-
?
N-formimino-L-alanine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-alanine + NH3
-
-
-
?
N-formimino-L-aspartate + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-aspartate + NH3
-
-
-
?
N-formimino-L-glutamate + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-glutamate + NH3
-
-
-
?
N-formimino-L-isoleucine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-isoleucine + NH3
-
-
-
?
N-formimino-L-leucine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-leucine + NH3
-
-
-
?
N-formimino-L-methionine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-methionine + NH3
-
-
-
?
N-formimino-L-phenylalanine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-phenylalanine + NH3
-
-
-
?
N-formimino-L-tyrosine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-tyrosine + NH3
-
-
-
?
N-formimino-L-valine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-valine + NH3
-
-
-
?
N-guanidino-L-glutamate + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 49000, SDS-PAGE, gel filtration
Pseudomonas aeruginosa
Synonyms
Synonyms
Commentary
Organism
N-formimino-L-glutamate deiminase
-
Pseudomonas aeruginosa
Pa5106
-
Pseudomonas aeruginosa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
less than 0.001/sec for the substrates N-guanidino-L-glutamate, N-formimino-alpha-aminobutyric acid, N-formimino-gamma-aminobutyric acid, N-formimino-L-alanine, N-formimino-L-aspartate, N-formimino-glycine, N-formimino-L-leucine, N-formimino-L-phenylalanine, N-formimino-L-tyrosine, N-formimino-L-valine
Pseudomonas aeruginosa
0.01
-
N-formimino-L-methionine
pH 8.0, 30°C
Pseudomonas aeruginosa
0.019
-
N-formimino-L-isoleucine
pH 8.0, 30°C
Pseudomonas aeruginosa
0.05
-
N-formimino-D-glutamate
pH 8.0, 30°C
Pseudomonas aeruginosa
8.5
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Cu2+, pH 8.0, 30°C
Pseudomonas aeruginosa
13.2
-
N-formimino-L-glutamate
pH 8.0, 30°C
Pseudomonas aeruginosa
21.3
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Ni2+, pH 8.0, 30°C
Pseudomonas aeruginosa
31
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Cd2+, pH 8.0, 30°C
Pseudomonas aeruginosa
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.03
-
N-formimino-L-aspartate
pH 8.0, 30°C
Pseudomonas aeruginosa
0.89
-
N-guanidino-L-glutamate
pH 8.0, 30°C
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by N-acetyl-L-glutamate, N-carbamoyl-L-glutamate, N-formyl-L-glutamate, N-formimino-alpha-aminobutyric acid and N-formimino-gamma-aminobutyric acid at concentrations up to 10 mM
Pseudomonas aeruginosa
N-formimino-L-aspartate
-
Pseudomonas aeruginosa
N-guanidino-L-glutamate
-
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.03
-
N-formimino-L-aspartate
pH 8.0, 30°C
Pseudomonas aeruginosa
0.89
-
N-guanidino-L-glutamate
pH 8.0, 30°C
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0012
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Cu2+, pH 8.0, 30°C
Pseudomonas aeruginosa
0.0021
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Cd2+, pH 8.0, 30°C
Pseudomonas aeruginosa
0.0027
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Ni2+, pH 8.0, 30°C
Pseudomonas aeruginosa
0.22
-
N-formimino-L-glutamate
wild-type, pH 8.0, 30°C
Pseudomonas aeruginosa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
bound to the protein
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
2 * 49000, SDS-PAGE, gel filtration
Pseudomonas aeruginosa
100000
-
gel filtration
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N-formimino-alpha-aminobutyric acid + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-alpha-aminobutyric acid + NH3
-
-
-
?
N-formimino-D-glutamate + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-D-glutamate + NH3
-
-
-
?
N-formimino-gamma-aminobutyric acid + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-gamma-aminobutyric acid + NH3
-
-
-
?
N-formimino-glycine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-glycine + NH3
-
-
-
?
N-formimino-L-alanine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-alanine + NH3
-
-
-
?
N-formimino-L-aspartate + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-aspartate + NH3
-
-
-
?
N-formimino-L-glutamate + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-glutamate + NH3
-
-
-
?
N-formimino-L-isoleucine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-isoleucine + NH3
-
-
-
?
N-formimino-L-leucine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-leucine + NH3
-
-
-
?
N-formimino-L-methionine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-methionine + NH3
-
-
-
?
N-formimino-L-phenylalanine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-phenylalanine + NH3
-
-
-
?
N-formimino-L-tyrosine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-tyrosine + NH3
-
-
-
?
N-formimino-L-valine + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
N-formyl-L-valine + NH3
-
-
-
?
N-guanidino-L-glutamate + H2O
pH 8.0, 30°C
664125
Pseudomonas aeruginosa
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 49000, SDS-PAGE, gel filtration
Pseudomonas aeruginosa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
less than 0.001/sec for the substrates N-guanidino-L-glutamate, N-formimino-alpha-aminobutyric acid, N-formimino-gamma-aminobutyric acid, N-formimino-L-alanine, N-formimino-L-aspartate, N-formimino-glycine, N-formimino-L-leucine, N-formimino-L-phenylalanine, N-formimino-L-tyrosine, N-formimino-L-valine
Pseudomonas aeruginosa
0.01
-
N-formimino-L-methionine
pH 8.0, 30°C
Pseudomonas aeruginosa
0.019
-
N-formimino-L-isoleucine
pH 8.0, 30°C
Pseudomonas aeruginosa
0.05
-
N-formimino-D-glutamate
pH 8.0, 30°C
Pseudomonas aeruginosa
8.5
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Cu2+, pH 8.0, 30°C
Pseudomonas aeruginosa
13.2
-
N-formimino-L-glutamate
pH 8.0, 30°C
Pseudomonas aeruginosa
21.3
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Ni2+, pH 8.0, 30°C
Pseudomonas aeruginosa
31
-
N-formimino-L-glutamate
Zn2+ has been reconstituted with Cd2+, pH 8.0, 30°C
Pseudomonas aeruginosa
Other publictions for EC 3.5.3.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733363
Fedorov
Structure of N-formimino-L-glu ...
Pseudomonas aeruginosa
Biochemistry
54
890-897
2015
-
-
-
1
-
-
2
-
-
1
-
1
-
4
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664125
Marti-Arbona
Annotating enzymes of unknown ...
Pseudomonas aeruginosa
Biochemistry
45
1997-2005
2006
-
-
1
-
-
-
3
4
-
1
2
-
-
4
-
-
1
-
-
-
-
-
14
1
2
-
-
-
8
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
3
2
4
-
1
2
-
-
-
-
1
-
-
-
-
14
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
685068
Marti-Arbona
Mechanistic characterization o ...
Pseudomonas aeruginosa
Biochemistry
45
14256-14262
2006
-
-
-
-
5
-
1
7
-
4
-
-
-
6
-
-
1
-
-
-
-
-
1
-
2
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
7
-
4
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
209406
Hu
Purification and properties of ...
Comamonas testosteroni, Pseudomonas aeruginosa, Pseudomonas putida, Streptomyces coelicolor
J. Bacteriol.
169
4696-4702
1987
-
-
1
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
9
-
8
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
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-
-
-
-
9
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
172087
Kendrick
Histidine dissimilation in Str ...
Streptomyces coelicolor
J. Gen. Microbiol.
128
2029-2040
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
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-
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209408
Wickner
N-Formimino-L-glutamate iminoh ...
Pseudomonas aeruginosa
J. Biol. Chem.
247
1605-1609
1972
-
-
-
-
-
-
1
1
-
-
6
1
-
3
-
-
1
-
-
-
1
-
3
3
-
-
-
-
-
-
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-
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-
1
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1
-
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6
1
-
-
-
1
-
-
1
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209409
Wickner
-
N-Formimino-L-glutamate iminoh ...
Pseudomonas aeruginosa
Methods Enzymol.
17
80-84
1971
-
-
-
-
-
-
-
-
-
-
2
1
-
1
-
-
1
-
-
-
1
1
2
1
-
-
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-
-
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-
-
-
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-
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-
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-
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2
1
-
-
-
1
-
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1
1
2
1
-
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-
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