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Literature summary for 3.5.3.12 extracted from

  • Shek, R.; Dattmore, D.A.; Stives, D.P.; Jackson, A.L.; Chatfield, C.H.; Hicks, K.A.; French, J.B.
    Structural and functional basis for targeting Campylobacter jejuni agmatine deiminase to overcome antibiotic resistance (2017), Biochemistry, 56, 6734-6742 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.12

Application

Application Comment Organism
drug development Campylobacter jejuni agmatine deiminase is a potentially important target for combatting antibiotic resistance Campylobacter jejuni subsp. jejuni serotype O:2

Cloned(Commentary)

Cloned (Comment) Organism
expression of the wild-type enzyme and the C315S mutant enzyme in Escherichia coli BL21 (DE3) Campylobacter jejuni subsp. jejuni serotype O:2

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of both the unliganded wild-type enzyme and a catalytic mutant of agmatine deiminase with the substrate, agamatine, bound Campylobacter jejuni subsp. jejuni serotype O:2

Protein Variants

Protein Variants Comment Organism
C315S enzymatically inactive form Campylobacter jejuni subsp. jejuni serotype O:2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 agmatine pH 7.0, 37°C Campylobacter jejuni subsp. jejuni serotype O:2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agmatine + H2O Campylobacter jejuni subsp. jejuni serotype O:2 the enzyme is involved in the biosynthesis of polyamines N-carbamoylputrescine + NH3
-
 ?
agmatine + H2O Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 the enzyme is involved in the biosynthesis of polyamines N-carbamoylputrescine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni subsp. jejuni serotype O:2 Q0P9V0
-
-
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 Q0P9V0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Campylobacter jejuni subsp. jejuni serotype O:2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 N-carbamoylputrescine + NH3
-
 ?
agmatine + H2O the enzyme is involved in the biosynthesis of polyamines Campylobacter jejuni subsp. jejuni serotype O:2 N-carbamoylputrescine + NH3
-
 ?
agmatine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 N-carbamoylputrescine + NH3
-
 ?
agmatine + H2O the enzyme is involved in the biosynthesis of polyamines Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 N-carbamoylputrescine + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00533
-
 agmatine pH 7.0, 37°C Campylobacter jejuni subsp. jejuni serotype O:2

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of polyamines Campylobacter jejuni subsp. jejuni serotype O:2