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Literature summary for 3.5.3.12 extracted from

  • Soares, C.A.; Knuckley, B.
    Mechanistic studies of the agmatine deiminase from Listeria monocytogenes (2016), Biochem. J., 473, 1553-1561 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Listeria monocytogenes serotype 1/2a

Inhibitors

Inhibitors Comment Organism Structure
2-chloroacetamidine
-
 Listeria monocytogenes serotype 1/2a
iodoacetamide
-
 Listeria monocytogenes serotype 1/2a
N-(4-aminobutyl)-2-chloro-ethanimidamide
-
 Listeria monocytogenes serotype 1/2a
N-(4-aminobutyl)-2-fluoro-ethanimidamide
-
 Listeria monocytogenes serotype 1/2a

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 agmatine pH 7.5, 25°C Listeria monocytogenes serotype 1/2a

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agmatine + H2O Listeria monocytogenes serotype 1/2a the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment N-carbamoylputrescine + NH3
-
 ?
agmatine + H2O Listeria monocytogenes serotype 1/2a 10403S the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment N-carbamoylputrescine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes serotype 1/2a A0A0H3G8U9
-
-
Listeria monocytogenes serotype 1/2a 10403S A0A0H3G8U9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Listeria monocytogenes serotype 1/2a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + H2O
-
 Listeria monocytogenes serotype 1/2a N-carbamoylputrescine + NH3
-
 ?
agmatine + H2O the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment Listeria monocytogenes serotype 1/2a N-carbamoylputrescine + NH3
-
 ?
agmatine + H2O
-
 Listeria monocytogenes serotype 1/2a 10403S N-carbamoylputrescine + NH3
-
 ?
agmatine + H2O the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment Listeria monocytogenes serotype 1/2a 10403S N-carbamoylputrescine + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 46800, SDS-PAGE Listeria monocytogenes serotype 1/2a

Synonyms

Synonyms Comment Organism
AgD
-
 Listeria monocytogenes serotype 1/2a

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.517
-
 agmatine pH 7.5, 25°C Listeria monocytogenes serotype 1/2a

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Listeria monocytogenes serotype 1/2a

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 pH 5.5: about 60% of maximal activity, pH 9.5: about 65% of maximal activity Listeria monocytogenes serotype 1/2a

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0785
-
 pH 7.5, 25°C Listeria monocytogenes serotype 1/2a N-(4-aminobutyl)-2-chloro-ethanimidamide
0.146
-
 pH 7.5, 25°C Listeria monocytogenes serotype 1/2a N-(4-aminobutyl)-2-fluoro-ethanimidamide
221
-
 pH 7.5, 25°C Listeria monocytogenes serotype 1/2a 2-chloroacetamidine

General Information

General Information Comment Organism
metabolism the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment Listeria monocytogenes serotype 1/2a

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.57
-
 agmatine pH 7.5, 25°C Listeria monocytogenes serotype 1/2a