Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Listeria monocytogenes |
Protein Variants | Comment | Organism |
---|---|---|
C157G | the mutant of inactive isoform AguA2 shows wild type enzyme activity | Listeria monocytogenes |
C356A | inactive | Listeria monocytogenes |
D218A | inactive | Listeria monocytogenes |
D218E | inactive | Listeria monocytogenes |
D94A | inactive | Listeria monocytogenes |
D94E | inactive | Listeria monocytogenes |
E155A | inactive | Listeria monocytogenes |
E155D | inactive | Listeria monocytogenes |
G157C | inactive | Listeria monocytogenes |
H216A | inactive | Listeria monocytogenes |
H216R | inactive | Listeria monocytogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Listeria monocytogenes | |
Cu2+ | - |
Listeria monocytogenes | |
additional information | no inhibitory effect on isoform AguA1 activity with Fe3+, Mg2+, and Ni2+ | Listeria monocytogenes | |
Zn2+ | - |
Listeria monocytogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
agmatine | at 25°C and pH 7.5 | Listeria monocytogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46800 | - |
x * 46800, calculated from amino acid sequence | Listeria monocytogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | Listeria monocytogenes | - |
N-carbamoylputrescine + NH3 | - |
? | |
agmatine + H2O | Listeria monocytogenes 10403S | - |
N-carbamoylputrescine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes | - |
- |
- |
Listeria monocytogenes 10403S | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelated agarose gel column chromatography | Listeria monocytogenes |
Storage Stability | Organism |
---|---|
25°C, purified enzyme, 6 h, 80% loss of activity | Listeria monocytogenes |
4°C, purified enzyme, at least 1 month, 15% loss of activity | Listeria monocytogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | - |
Listeria monocytogenes | N-carbamoylputrescine + NH3 | - |
? | |
agmatine + H2O | - |
Listeria monocytogenes 10403S | N-carbamoylputrescine + NH3 | - |
? | |
additional information | L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity | Listeria monocytogenes | ? | - |
? | |
additional information | L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity | Listeria monocytogenes 10403S | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46800, calculated from amino acid sequence | Listeria monocytogenes |
Synonyms | Comment | Organism |
---|---|---|
AgDI | - |
Listeria monocytogenes |
AguA1 | - |
Listeria monocytogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Listeria monocytogenes |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 35 | about 65% activity at 5°C, about 90% activity at 15°C, 100% activity at 25°C, less than 40% activity at 35°C | Listeria monocytogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34.28 | - |
agmatine | at 25°C and pH 7.5 | Listeria monocytogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Listeria monocytogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10.5 | more than 85% activity between pH 3.5 and 10.5 | Listeria monocytogenes |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.034 | - |
at 25°C and pH 7.5 | Listeria monocytogenes | Cu2+ | |
0.25 | - |
at 25°C and pH 7.5 | Listeria monocytogenes | Zn2+ | |
2.89 | - |
at 25°C and pH 7.5 | Listeria monocytogenes | Co2+ |
Organism | Comment | Expression |
---|---|---|
Listeria monocytogenes | transcription is significantly induced at pH 5.0 | up |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of aguA1 significantly impairs Listeria monocytogenes survival both in gastric fluid at pH 2.5 and in mouse stomach | Listeria monocytogenes |
physiological function | the enzyme mediates acid tolerance in Listeria monocytogenes | Listeria monocytogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
53 | - |
agmatine | at 25°C and pH 7.5 | Listeria monocytogenes |