BRENDA - Enzyme Database
show all sequences of 3.5.3.12

Factors affecting the production of putrescine from agmatine by Lactobacillus hilgardii XB isolated from wine

Arena, M.E.; Landete, J.M.; Manca de Nadra, M.C.; Pardo, I.; Ferrer, S.; J. Appl. Microbiol. 105, 158-165 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
L-lactic acid
shows a stimulatory effect on activity
Lactobacillus hilgardii
additional information
ornithine, D-lactic acid, citric acid, L-malic acid, and DL-malic acid do not modify activity
Lactobacillus hilgardii
tartaric acid
shows a stimulatory effect on activity
Lactobacillus hilgardii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus hilgardii
-
-
-
Lactobacillus hilgardii XB
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
agmatine + H2O
-
687349
Lactobacillus hilgardii
N-carbamoylputrescine + NH3
-
-
-
ir
agmatine + H2O
-
687349
Lactobacillus hilgardii XB
N-carbamoylputrescine + NH3
-
-
-
ir
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
-
Lactobacillus hilgardii
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
above 40C a noticeable diminution of activity is observed
Lactobacillus hilgardii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
-
Lactobacillus hilgardii
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
2.5
6
agmatine is degraded in a very limited extent when the pH values are close to 2.5, a diminution of activity is observed at pH values above 6.0, at pH values from 3.2 to 3.8, the amount of putrescine formed is 53% and 75% respectively of the maximum amount formed at pH 4.5
Lactobacillus hilgardii
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
L-lactic acid
shows a stimulatory effect on activity
Lactobacillus hilgardii
additional information
ornithine, D-lactic acid, citric acid, L-malic acid, and DL-malic acid do not modify activity
Lactobacillus hilgardii
tartaric acid
shows a stimulatory effect on activity
Lactobacillus hilgardii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
agmatine + H2O
-
687349
Lactobacillus hilgardii
N-carbamoylputrescine + NH3
-
-
-
ir
agmatine + H2O
-
687349
Lactobacillus hilgardii XB
N-carbamoylputrescine + NH3
-
-
-
ir
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
-
Lactobacillus hilgardii
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
above 40C a noticeable diminution of activity is observed
Lactobacillus hilgardii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
-
Lactobacillus hilgardii
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
2.5
6
agmatine is degraded in a very limited extent when the pH values are close to 2.5, a diminution of activity is observed at pH values above 6.0, at pH values from 3.2 to 3.8, the amount of putrescine formed is 53% and 75% respectively of the maximum amount formed at pH 4.5
Lactobacillus hilgardii
Other publictions for EC 3.5.3.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733200
del Rio
Putrescine production via the ...
Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. cremoris CECT 8666
Appl. Microbiol. Biotechnol.
99
897-905
2015
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1
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4
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3
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1
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4
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1
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4
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-
-
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-
-
1
1
-
-
-
733510
Marchenko
Development of a clickable act ...
Streptococcus mutans, Streptococcus mutans UA159
Bioorg. Med. Chem.
23
2159-2167
2015
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1
2
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18
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1
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2
1
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734228
Cheng
Listeria monocytogenes aguA1, ...
Listeria monocytogenes 10403S, Listeria monocytogenes
J. Biol. Chem.
288
26606-26615
2013
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-
1
-
11
-
4
1
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1
2
-
8
-
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1
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2
4
1
1
1
-
1
1
1
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3
-
-
1
-
-
11
-
3
4
-
1
-
-
1
2
-
-
-
1
-
-
-
2
4
1
1
1
-
1
1
1
-
-
1
2
2
1
1
1
734787
Chen
Prevalence of the lmo0036-0043 ...
Listeria monocytogenes
New Microbiol.
36
187-192
2013
-
-
-
-
-
-
-
-
-
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1
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2
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
735102
Suarez
Expression of the agmatine dei ...
Enterococcus faecalis, Enterococcus faecalis JH2-2
PLoS ONE
8
e76170
2013
1
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
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2
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1
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2
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-
2
-
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-
-
-
-
-
-
-
1
-
-
1
-
-
711375
Jones
Characterization and inactivat ...
Helicobacter pylori
Bioorg. Chem.
38
62-73
2010
-
-
1
1
-
-
5
2
-
-
-
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-
3
-
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8
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2
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5
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1
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1
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5
5
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2
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-
-
-
8
-
-
-
-
2
-
-
-
-
-
-
-
-
16
16
712057
Coton
Occurrence of biogenic amine-f ...
Bacteria
Food Microbiol.
27
1078-1085
2010
-
2
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713057
Williams
Discovery of an operon that pa ...
Pseudomonas aeruginosa PA14
Mol. Microbiol.
76
104-119
2010
-
-
1
-
2
-
-
-
1
-
1
-
-
4
-
1
-
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
2
1
2
2
2
2
718884
Jones
Mechanistic studies of agmatin ...
Helicobacter pylori, Porphyromonas gingivalis, Streptococcus mutans
Biochemistry
49
9413-9423
2010
-
-
-
-
-
-
12
-
-
-
-
-
-
8
-
-
3
3
-
-
-
-
3
-
3
-
-
-
3
-
-
-
-
-
6
-
-
-
-
-
-
-
6
12
-
-
-
-
-
-
-
-
-
3
-
-
-
-
3
-
3
-
-
-
3
-
-
-
-
-
-
-
-
-
719620
Landete
The role of two families of ba ...
Bacillus cereus, Bacillus cereus ATCC 14579, Enterococcus faecalis, Enterococcus faecalis ATCC 11700, Lactobacillus hilgardii, Lactobacillus hilgardii X1B, Lactobacillus sakei, Pseudomonas aeruginosa
Int. Microbiol.
13
169-177
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
23
-
-
-
-
-
-
-
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-
-
5
-
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5
-
-
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-
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-
-
-
-
-
-
-
5
-
-
-
5
-
-
-
-
-
-
-
-
-
695768
Liu
AguR is required for induction ...
Streptococcus mutans
Appl. Environ. Microbiol.
75
2629-2637
2009
1
-
-
-
-
-
-
-
-
-
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-
2
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1
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1
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1
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-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
700535
Griswold
Distribution, regulation and r ...
no activity in Streptococcus gordonii, no activity in Streptococcus sanguinis, Streptococcus criceti, Streptococcus criceti AHT, Streptococcus downei, Streptococcus downei 33748, Streptococcus mutans, Streptococcus oralis, Streptococcus ratti, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus sobrinus, Streptococcus sobrinus 6715
Oral Microbiol. Immunol.
24
79-82
2009
5
5
-
-
-
-
1
-
-
-
-
-
-
18
-
-
-
-
-
-
18
-
11
-
-
-
-
-
8
-
-
-
-
-
-
5
5
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
18
-
11
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
712291
Liu
Multiple two-component systems ...
Streptococcus mutans
J. Bacteriol.
191
7363-7366
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
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-
-
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-
-
-
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-
-
1
-
-
1
-
-
685687
Liao
Occurrence of agmatine pathway ...
Selenomonas ruminantium
Biosci. Biotechnol. Biochem.
72
445-455
2008
-
-
1
-
1
-
9
1
-
-
1
-
-
5
-
-
1
-
-
-
-
-
1
1
1
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
1
-
-
9
-
1
-
-
1
-
-
-
-
1
-
-
-
-
1
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
687349
Arena
Factors affecting the producti ...
Lactobacillus hilgardii, Lactobacillus hilgardii XB
J. Appl. Microbiol.
105
158-165
2008
3
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
1
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1
-
1
-
1
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3
-
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-
-
2
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
692430
Landete
Comparative survey of putresci ...
Bacillus cereus, Bacillus cereus CECT 148, Enterococcus faecalis, Enterococcus faecalis ATCC 11700, Lactobacillus hilgardii, Lactobacillus hilgardii X1B, Pseudomonas aeruginosa
Food Microbiol.
25
882-887
2008
9
-
-
-
-
-
20
-
-
-
-
1
-
15
-
-
-
-
-
-
1
-
9
-
4
-
-
-
4
-
-
-
-
-
-
9
-
-
-
-
-
-
-
20
-
-
-
-
-
1
-
-
-
-
-
-
1
-
9
-
5
-
-
-
5
-
-
-
-
-
-
-
-
-
674316
Llacer
The gene cluster for agmatine ...
Enterococcus faecalis, Enterococcus faecalis ATCC 700802
J. Bacteriol.
189
1254-1265
2007
-
-
1
1
-
-
1
-
-
-
1
-
-
4
-
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1
-
-
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4
1
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1
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1
-
1
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1
1
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1
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1
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-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669131
Griswold
Regulation and physiologic sig ...
Streptococcus mutans, Streptococcus mutans UA159
J. Bacteriol.
188
834-841
2006
-
-
1
-
-
-
1
-
-
-
-
-
-
20
-
-
-
-
-
-
-
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2
-
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1
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-
1
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1
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1
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-
-
-
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
653166
Nakada
Identification of the putresci ...
Pseudomonas aeruginosa
Microbiology
149
707-714
2003
-
-
1
-
1
-
3
1
-
-
3
-
-
3
-
-
1
-
-
-
-
-
2
1
1
-
-
1
1
-
-
-
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1
-
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1
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3
-
1
-
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3
-
-
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-
1
-
-
-
-
2
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
655571
Janowitz
Identification and characteriz ...
Arabidopsis thaliana
FEBS Lett.
544
258-261
2003
-
-
1
-
2
-
2
-
-
-
2
-
-
4
-
-
1
1
-
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1
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2
1
1
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-
1
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1
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2
-
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2
-
-
-
-
2
-
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657259
Sakakibara
Agmatine deiminase from cucumb ...
Cucumis sativus
Protein Expr. Purif.
30
88-93
2003
-
-
-
-
-
-
1
1
-
-
3
-
-
4
-
1
1
1
-
2
1
1
1
1
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
3
-
-
-
1
1
-
2
1
1
1
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
669081
Nakada
Molecular characterization and ...
Arabidopsis thaliana, Campylobacter jejuni, Caulobacter vibrioides, Chlorella virus, Deinococcus radiodurans, Helicobacter pylori, Lactococcus lactis, Pseudomonas aeruginosa, Streptomyces coelicolor, Xylella fastidiosa
J. Bacteriol.
183
6517-6524
2001
-
-
1
-
1
-
-
-
-
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1
-
-
14
-
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-
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1
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-
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-
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1
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1
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1
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-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670510
Yanagisawa
Agmatine deiminase from maize ...
Zea mays
Phytochemistry
56
643-647
2001
-
-
-
-
-
-
1
1
-
-
3
-
-
2
-
-
1
-
-
2
3
-
1
1
-
-
1
-
1
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
3
-
-
-
-
1
-
2
3
-
1
1
-
-
1
-
1
2
-
-
-
-
-
-
-
-
209405
Shoeb
-
Differential enzymatic measure ...
Rhodococcus sp., Rhodococcus sp. C-x
Biosci. Biotechnol. Biochem.
60
69-72
1996
-
2
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
4
-
-
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-
-
-
-
-
-
-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209404
Park
Purification of monomeric agma ...
Glycine max, Oryza sativa, Zea mays
Biochem. Biophys. Res. Commun.
174
32-36
1991
-
-
-
-
-
-
14
3
3
-
3
3
-
6
-
-
3
-
-
4
1
2
5
3
3
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
3
3
-
3
3
-
-
-
3
-
4
1
2
5
3
3
-
-
-
3
-
-
-
-
-
-
-
-
-
209403
Prasad
-
Purification and characterizat ...
Cucumis sativus, Lathyrus sativus
J. Biosci.
10
373-391
1986
-
-
-
-
-
1
-
-
-
-
5
2
-
2
-
-
2
-
-
3
1
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
5
2
-
-
-
2
-
3
1
-
7
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209402
Chaudhuri
-
Agmatine deiminase in rice see ...
Arachis hypogaea, Helianthus annuus, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Zea mays
Phytochemistry
24
2433-2435
1985
-
-
-
-
-
-
13
1
-
-
2
6
-
6
-
-
2
-
-
7
1
1
6
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
1
-
-
2
6
-
-
-
2
-
7
1
1
6
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
209401
Legaz
-
Regulation of agmatine iminohy ...
Evernia prunastri, Glycine max, Hordeum vulgare, Lathyrus sativus, Soja hispida, Zea mays
Z. Pflanzenphysiol.
110
53-59
1983
1
-
-
-
-
-
6
1
-
-
-
6
-
6
-
-
1
-
-
1
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
1
-
-
-
6
-
-
-
1
-
1
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209400
Simon
-
Enzymes of agmatine degradatio ...
Enterococcus faecalis
J. Bacteriol.
11
676-681
1982
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209398
Yanagisawa
Corn agmatine iminohydrolase. ...
Arachis hypogaea, Helianthus annuus, Zea mays
Plant Physiol.
67
697-700
1981
-
-
-
-
-
-
17
2
1
-
2
3
-
4
-
-
2
-
-
6
1
1
3
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
17
-
2
1
-
2
3
-
-
-
2
-
6
1
1
3
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
209399
Srivenugopal
Enzymic conversion of agmatine ...
Lathyrus sativus
J. Biol. Chem.
256
9532-9541
1981
-
-
-
-
-
1
-
-
-
-
2
1
-
2
-
-
1
-
-
1
1
1
6
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
1
-
-
-
1
-
1
1
1
6
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
209395
Le Rudulier
-
Biogenese de la N-carbamylputr ...
Glycine max, Zea mays
Physiol. Veg.
18
609-616
1980
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
209397
Sindhu
-
Purification and properties of ...
Arachis hypogaea, Escherichia coli, Helianthus annuus, Zea mays
Phytochemistry
18
1937-1938
1979
-
-
-
-
-
-
13
1
-
-
-
4
-
4
-
-
1
-
-
6
1
-
4
-
-
-
1
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
13
2
1
-
-
-
4
-
-
-
1
-
6
1
-
4
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
209396
Smith
-
Agmatine iminohydrolase in mai ...
Avena sativa, Brassica oleracea, Helianthus annuus, Hordeum vulgare, no activity in Malus sylvestris, no activity in Pisum sativum, no activity in Secale cereale, no activity in Triticum aestivum, Zea mays
Phytochemistry
8
2111-2117
1969
-
-
-
-
-
-
4
-
-
-
-
5
-
9
-
-
-
-
-
5
-
-
5
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
5
-
-
-
-
-
5
-
-
5
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-