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Literature summary for 3.5.3.12 extracted from
- Occurrence of agmatine pathway for putrescine synthesis in Selenomonas ruminatium (2008), Biosci. Biotechnol. Biochem., 72, 445-455.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain DE3 | Selenomonas ruminantium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C361A | 20% enzyme activity of wild-type enzyme | Selenomonas ruminantium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 1 mM | Selenomonas ruminantium |  |
| Co2+ | 1 mM | Selenomonas ruminantium | |
| Cu2+ | 1 mM | Selenomonas ruminantium | |
| Fe2+ | 1 mM | Selenomonas ruminantium | |
| Fe3+ | 1 mM | Selenomonas ruminantium | |
| Hg2+ | strong inhibitor | Selenomonas ruminantium | |
| iodoacetamide | strong inhibitor | Selenomonas ruminantium | |
| Ni2+ | 1 mM | Selenomonas ruminantium | |
| Zn2+ | 1 mM | Selenomonas ruminantium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.67 | - |
agmatine | - |
Selenomonas ruminantium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration | Selenomonas ruminantium |
| 45000 | - |
SDS-PAGE | Selenomonas ruminantium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Selenomonas ruminantium | Q5KR05 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Selenomonas ruminantium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | 25 mM, assay at pH 6.5, 10 min, 40°C | Selenomonas ruminantium | putrescine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Selenomonas ruminantium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| agmatine deiminase | - |
Selenomonas ruminantium |
| AguA | - |
Selenomonas ruminantium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Selenomonas ruminantium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
below 40°C stable for 30 min, pH 6.0-9.0 | Selenomonas ruminantium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Selenomonas ruminantium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 30 min, temperature below 40°C | Selenomonas ruminantium |
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Release 2023.1 (January 2023)
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