Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arcaine | agmatine analogue, strong competitive inhibition | Cucumis sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
agmatine | pH 7.0, 30°C | Cucumis sativus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, deglycosylated enzyme, SDS-PAGE, x * 47000, glycosylated enzyme, SDS-PAGE, monomer or dimer | Cucumis sativus |
47000 | - |
x * 36000, deglycosylated enzyme, SDS-PAGE, x * 47000, glycosylated enzyme, SDS-PAGE, monomer or dimer | Cucumis sativus |
67000 | - |
gel filtration | Cucumis sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucumis sativus | - |
cv. Tokiwajibae | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation | Cucumis sativus |
Purification (Comment) | Organism |
---|---|
2470fold | Cucumis sativus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
agmatine + H2O = N-carbamoylputrescine + NH3 | the enzyme does not show activity of EC 2.1.3.3, ornithine carbamoyltransferase, EC 2.1.3.6, putrescine carbamoyltransferase, and EC 2.7.2.2, carbamate kinase, while putrescine synthase is a multifunctional enzyme, overview | Cucumis sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Cucumis sativus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.5 | - |
purified enzyme | Cucumis sativus |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, 2 months, stable | Cucumis sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | - |
Cucumis sativus | N-carbamoylputrescine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | x * 36000, deglycosylated enzyme, SDS-PAGE, x * 47000, glycosylated enzyme, SDS-PAGE, monomer or dimer | Cucumis sativus |
Synonyms | Comment | Organism |
---|---|---|
agmatine amidinohydrolase | - |
Cucumis sativus |
agmatine deiminase | - |
Cucumis sativus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cucumis sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cucumis sativus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | 89% of maximal activity at pH 6.5, 91% of maximal activity at pH 8.0 | Cucumis sativus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0071 | - |
arcaine | pH 7.0, 30°C | Cucumis sativus |