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Literature summary for 3.5.3.12 extracted from
- Corn agmatine iminohydrolase. Purification and properties (1981), Plant Physiol., 67, 697-700.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Zea mays |  |
| cadaverine | - |
Arachis hypogaea | |
| Co2+ | 1 mM 55% inhibition | Zea mays | |
| Cu2+ | - |
Arachis hypogaea | |
| Cu2+ | 1 mM 100% inhibition | Zea mays | |
| Fe2+ | - |
Zea mays | |
| Mg2+ | - |
Zea mays | |
| N,N'-diguanidinobutane | - |
Zea mays | |
| Ni2+ | - |
Zea mays | |
| p-chloromercuribenzoate | - |
Arachis hypogaea | |
| p-chloromercuribenzoate | - |
Zea mays | |
| putrescine | - |
Arachis hypogaea | |
| spermidine | - |
Arachis hypogaea | |
| spermine | - |
Arachis hypogaea | |
| tryptamine | - |
Arachis hypogaea | |
| Zn2+ | - |
Arachis hypogaea | |
| Zn2+ | 1 mM 85% inhibition | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
agmatine | - |
Zea mays | |
| 0.76 | - |
agmatine | - |
Arachis hypogaea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Zea mays | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, SDS-PAGE | Zea mays |
| 85000 | - |
gel filtration | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | Zea mays | - |
N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | Arachis hypogaea | - |
N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | Helianthus annuus | - |
N-carbamoylputrescine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arachis hypogaea | - |
- |
- |
| Helianthus annuus | - |
- |
- |
| Zea mays | - |
L. cv Goldencross Bantam T51 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
- |
Arachis hypogaea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Arachis hypogaea | - |
| seedling | - |
Zea mays | - |
| seedling | - |
Helianthus annuus | - |
| shoot | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 876.3 | - |
- |
Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme lost 30% of the activity in 3 days | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | - |
Zea mays | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | - |
Arachis hypogaea | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | - |
Helianthus annuus | N-carbamoylputrescine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, SDS-PAGE | Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
exposure for 10 min, decrease of 50% in the activity | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | activity rapidly declining below pH 6.0 and declining less rapidly above pH 7.0 | Zea mays |
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