Application | Comment | Organism |
---|---|---|
medicine | inhibition of Leishmania arginase leads to a decrease in parasite growth and infectivity and thus represents an attractive therapeutic strategy | Leishmania infantum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) cells | Leishmania infantum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
apigenin 7-O-beta-D-glucoside | 0.1 mM, about 40% inhibition | Leishmania infantum | |
caffeic acid | 0.1 mM, 56.98% inhibition | Leishmania infantum | |
catechin | 0.1 mM, about 40% inhibition | Leishmania infantum | |
chlorogenic acid | 0.1 mM, about 30% inhibition | Leishmania infantum | |
dihydroquercetin | 0.1 mM, about 25% inhibition | Leishmania infantum | |
eugenol | 0.1 mM, about 20% inhibition | Leishmania infantum | |
fungizone | - |
Leishmania infantum | |
isorhamnetin | 0.1 mM, about 55% inhibition | Leishmania infantum | |
quercetin | 0.1 mM, about 70% inhibition | Leishmania infantum | |
Rhamnetin | 0.1 mM, about 50% inhibition | Leishmania infantum | |
rhaponticin | 0.1 mM, about 60% inhibition | Leishmania infantum | |
rosmarinic acid | 0.1 mM, 71.48% inhibition | Leishmania infantum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.1 | - |
L-arginine | pH 9.5, 37°C | Leishmania infantum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | Leishmania infantum | - |
L-ornithine + urea | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania infantum | A0A145YEM9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leishmania infantum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | intracellular | Leishmania infantum | - |
promastigote | - |
Leishmania infantum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Leishmania infantum | L-ornithine + urea | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | - |
Leishmania infantum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | 25°C: about 40% of maximal activity, 65°C: about 45% of maximal activity | Leishmania infantum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Leishmania infantum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.6 | 10 | pH 8.6: about 55% of maximal activity, pH 10.0: about 60% of maximal activity | Leishmania infantum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00005 | - |
pH 9.5, 37°C, activity in promastigote | Leishmania infantum | fungizone | |
0.0079 | - |
pH 9.5, 37°C, activity in intracellular amastigote | Leishmania infantum | rosmarinic acid | |
0.0219 | - |
pH 9.5, 37°C, activity in intracellular amastigote | Leishmania infantum | caffeic acid | |
0.0573 | - |
pH 9.5, 37°C, activity in promastigote | Leishmania infantum | rosmarinic acid | |
0.0608 | - |
pH 9.5, 37°C, activity in promastigote | Leishmania infantum | caffeic acid | |
0.287 | - |
pH 9.5, 37°C, activity in intracellular amastigote | Leishmania infantum | catechin | |
0.395 | - |
pH 9.5, 37°C, activity in promastigote | Leishmania infantum | catechin | |
0.818 | - |
pH 9.5, 37°C, activity in promastigote | Leishmania infantum | isorhamnetin | |
0.832 | - |
pH 9.5, 37°C, activity in promastigote | Leishmania infantum | Rhamnetin |